{"product_id":"biolegend-629851","title":"Biolegend, 629851, Purified anti-Caldesmon Antibody, 25μg","description":"\u003cp\u003eCaldesmon is a calmodulin-, actin-, and tropomyosin-binding protein that plays an essential role in smooth muscle and non-muscle contraction. This protein is also involved in cellular mitosis and Schwann cell migration during peripheral nerve regeneration.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant fragment of human Caldesmon\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg\/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: For immunohistochemistry (IHC-P), either Citrate Buffer, 10X (Cat. No, 420902) or Tris-EDTA pH 9.0 is recommended for antigen retrieval. For immunocytochemistry (ICC), fixing with Fixation Buffer (Cat. No. 420801) is recommended. Alternatively, 4% paraformaldehyde fixation followed by either permeabilization with 0.5% Triton X-100 or ice-cold methanol is recommended. Clone W22071A cross-reacts with mouse in western blotting (WB).\u003cbr\u003e\nRRID: AB_3083418 (BioLegend Cat. No. 629851) AB_3083418 (BioLegend Cat. No. 629852)\u003cbr\u003e\nStructure: Caldesmon is a 793 amino acid protein with a predicted molecular mass of 93 kD\u003cbr\u003e\nDistribution: Tissue distribution: high-molecular-weight Caldesmon (isoform 1) is predominantly expressed in smooth muscle; low-molecular-weight Caldesmon (isoforms 2, 3, 4 and 5) is widely distributed in non-muscle tissues and cells. Caldesmon is not expressed in skeletal muscle or heart. Cellular distribution: cytoskeleton, cytosol, and plasma membrane.\u003cbr\u003e\nFunction: Regulates actomyosin interactions in smooth muscle and non-muscle cells which affects cell contractions; involved in cellular mitosis and in Schwann cell migration during peripheral nerve regeneration.\u003cbr\u003e\nInteraction: Calmodulin, actin, and tropomyosin\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: Huber PA. 1997. Int J Biochem Cell Biol. 29:1047-51. Mayanagi T and Sobue K. 2011. Cell. Adh. Migr. 5:150-9. Wang CL. 2008. Adv Exp Med Biol. 644:250-72. Sobue K and Sellers JR. 1991. J Biol Chem. 266:12115-8.\u003cbr\u003e\nGene ID: 800\u003cbr\u003e\nUniProt: View information about Caldesmon on UniProt.org\u003cbr\u003e\nClone: W22071A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Caldesmon 1, Caldesmon, CDM, H-CAD, L-CAD, H-CD, Testis Secretory Sperm-Binding Protein Li 227n, NAG22, LCAD, HCAD, CAD\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866432458921,"sku":"629851","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-629851","provider":"Iright","version":"1.0","type":"link"}