{"product_id":"biolegend-630301","title":"Biolegend, 630301, Purified anti-AND-1 (WDHD1) Antibody, 25μg","description":"\u003cp\u003eThe 20G10 monoclonal antibody recognizes both human and mouse AND-1 (WDHD1) also known as WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1. AND-1 (WDHD1) is a nuclear and nucleolar protein that contains multiple WD40 repeats, and one HMG region. AND-1 (WDHD1) has a predicted molecular weight of 126 kD and can form homodimers. WDHD1 is expressed in the brain, epidermis, liver, and stomach. During mitosis, AND-1 (WDHD1) is transiently expressed in nucleoplasm. AND-1 (WDHD1) can bind to DNA with high affinity and may be involved in protein-protein interactions. The role of AND-1 (WDHD1) is thought to be chromatin assembly, transcription and DNA replication. AND-1 (WDHD1) has been reported to be phosphorylated on S1041. The 20G10 antibody has been shown to be useful for Western blotting.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant (partial), C-terminal\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedIHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 15 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Prakash Yadav R, et al. 2023. Reproduction. 165:49. PubMed Kim B, et al. 2020. Molecular Cell. 78(4):624-640. PubMed Jeong DS, et al. 2022. Cell J. 24:51. PubMed\u003cbr\u003e\nRRID: AB_2215084 (BioLegend Cat. No. 630301) AB_2215083 (BioLegend Cat. No. 630302)\u003cbr\u003e\nStructure: Contains multiple WD40 repeats, one HMG region. Predicted molecular weight 126 kD. Forms homodimers.\u003cbr\u003e\nDistribution: Found in the nucleus and nucleolus. Expressed in brain, epidermis, liver, and stomach. During mitosis transiently expressed in nucleoplasm.\u003cbr\u003e\nFunction: Binds DNA with high affinity; may be involved in protein-protein interactions. Thought to function in chromatin assembly, transcription and DNA replication.\u003cbr\u003e\nModification: Phosphorylation (S1041)\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Kohler A, et al. 1997. J. Cell Sci. 110:1051.\u003cbr\u003e\nGene ID: 11169\u003cbr\u003e\nUniProt: View information about AND-1 on UniProt.org\u003cbr\u003e\nClone: 20G10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860868812969,"sku":"630301","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-630301","provider":"Iright","version":"1.0","type":"link"}