{"product_id":"biolegend-635402","title":"Biolegend, 635402, Purified anti-TIMP-2 Antibody, 100μg","description":"\u003cp\u003eTIMP-2 (Tissue inhibitor of metalloproteinase 2) is a nonglycosylated protein that belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain, predicted molecular weight 24 kD. The mechanisms of TIMPs inhibit angiogenesis appear to be specific for each member of the TIMP family. TIMP-2 binds to the surface of human endothelial cells through interaction with the integrin α3β1, and abrogates angiogenic factor induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. Clone F27P3A4 has been shown to be useful for western blotting, immunohistochemistry and ELISA of human, mouse, rabbit, chicken, zebrafish, and pig TIMP-2.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Mouse, Rabbit, Chicken, Zebrafish, Pig\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Ovalbumin-conjugated synthetic Peptide DSGNDIYGNPI\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P, ELISA - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Murray G et al. 1998. Gut. 43:791. Fromique O, et al. 2008. J. Biol. Chem. 283:30549. PubMed\u003cbr\u003e\nProduct Citations: Hrabia A, et al. 2022. Poult Sci. 101:101915. PubMed Fromigué O, et al. 2008. J Biol Chem. 283:30549. PubMed\u003cbr\u003e\nRRID: AB_2287490 (BioLegend Cat. No. 635401) AB_2204826 (BioLegend Cat. No. 635402)\u003cbr\u003e\nStructure: Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain, predicted molecular weight 24 kD.\u003cbr\u003e\nDistribution: Extracellular\u003cbr\u003e\nFunction: TIMP-2 binds to the surface of human endothelial cells through interaction with the integrin α3β1, and abrogates angiogenic factor induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition.\u003cbr\u003e\nInteraction: MMP2, MMP8, MMP14, CD49C\u003cbr\u003e\nBiology Area: Angiogenesis, Apoptosis\/Tumor Suppressors\/Cell Death, Cell Adhesion, Cell Biology\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: 1. Stetler-Stevenson, et al. 1989. J. Biol. Chem. 264:17374\u003cbr\u003e\nGene ID: 7077\u003cbr\u003e\nUniProt: View information about TIMP-2 on UniProt.org\u003cbr\u003e\nClone: F27P3A4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Metalloproteinase inhibitor 2, Tissue inhibitor of metalloproteinases 2, TIMP2\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863080685737,"sku":"635402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-635402","provider":"Iright","version":"1.0","type":"link"}