{"product_id":"biolegend-642152","title":"Biolegend, 642152, Purified anti-CUL3 Antibody, 100μg","description":"\u003cp\u003eCUL3 (Cullin-3) is a key component of the ubiquitin-proteasome system (UPS), an essential pathway for regulating protein degradation in human cells. It functions as part of an E3 ubiquitin ligase complex, which tags specific proteins with ubiquitin molecules, marking them for degradation by the proteasome. CUL3 is a member of the Cullin family of proteins, which provide a structural scaffold for the assembly of multi-subunit ubiquitin ligases, termed Cullin-RING ligases (CRLs). These ligases play a central role in maintaining protein homeostasis by controlling the turnover of numerous regulatory proteins involved in critical cellular processes such as cell cycle progression, signal transduction, transcription, and stress responses.  CUL3 forms a complex with a RING-domain protein, RBX1, which recruits the E2 ubiquitin-conjugating enzyme, and various substrate adaptors known as BTB domain-containing proteins. The BTB proteins determine substrate specificity, allowing CUL3 to target a wide range of proteins for degradation. This ability to selectively degrade proteins makes CUL3 a crucial regulator of several physiological and pathological processes.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant fragment of human CUL3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody (clone W21054D), is not recommended for use in either immunocytochemistry (ICC) or immunohistochemistry (IHC-P).\u003cbr\u003e\nRRID: AB_3675176 (BioLegend Cat. No. 642151) AB_3675176 (BioLegend Cat. No. 642152)\u003cbr\u003e\nStructure: CUL3 is a 768 amino acid length protein with a predicted molecular weight of 88.9 kD.\u003cbr\u003e\nDistribution: Located in nucleus, Golgi apparatus, and cytoplasm\u003cbr\u003e\nFunction: Mediates the ubiquitination and subsequent proteasomal degradation of target proteins\u003cbr\u003e\nInteraction: A component of multiple BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes\u003cbr\u003e\nBiology Area: Cell Proliferation and Viability, Ubiquitin\/Protein Degradation\u003cbr\u003e\nMolecular Family: Adaptor Proteins, Enzymes and Regulators\u003cbr\u003e\nAntigen References: Canning P, et al, 2013, J Biol Chem.288:7803-7814. McGourty C, et al. 2016. Cell. 167:525-538.e14. Dong Z, et al. 2019. Neuron. 105:475-490.e6.\u003cbr\u003e\nGene ID: 8452\u003cbr\u003e\nUniProt: View information about CUL3 on UniProt.org\u003cbr\u003e\nClone: W21054D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Cullin-3, CUL-3, Cullin3, PHA2E, KIAA0617\u003cbr\u003e\nIsotype: Rat IgG2b, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866428625065,"sku":"642152","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-642152","provider":"Iright","version":"1.0","type":"link"}