{"product_id":"biolegend-642352","title":"Biolegend, 642352, Purified anti-BLK Antibody, 100μg","description":"\u003cp\u003eBLK is a non-receptor tyrosine kinase primarily expressed in B lymphocytes and other cells of the B-cell lineage. BLK belongs to the Src family of kinases and plays a critical role in B cell receptor signaling, which is essential for the activation, differentiation, and proliferation of B cells. Phosphorylation of target proteins by BLK helps transmit signals that guide B cell development and activation. Dysregulation of BLK has been associated with various immune disorders, including autoimmune diseases and certain types of B cell malignancies. Outside of B cells, BLK is also expressed in a variety of immune and non-immune cells including plasmacytoid dendritic cells, pancreatic β cells, and bone marrow progenitor cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant full-length human BLK\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This product (clone W21005C) may cross-react with other members of the Src kinase family. This product (clone W21005C) is not recommended for use in immunocytochemistry (ICC) or immunohistochemistry (IHC). For use in intracellular flow cytometry (ICFC), permeabilization with True-PhosTM Perm Buffer (Cat. No. 425401) is recommended. Fixation\/permeabilization with Cyto-FastTM Fix\/Perm Buffer Set (Cat. No. 426803) is not recommended. This antibody does not cross react with mouse BLK in Western Blot (WB).\u003cbr\u003e\nRRID: AB_3675177 (BioLegend Cat. No. 642351) AB_3675177 (BioLegend Cat. No. 642352)\u003cbr\u003e\nStructure: BLK is a 505 amino acid protein with a predicted molecular weight of 57.7 kD.\u003cbr\u003e\nDistribution: Cytoplasmic, plasma membrane\u003cbr\u003e\nFunction: Kinase\u003cbr\u003e\nInteraction: Phosphorylates a variety of targets including BLNK, IRF3, SYK, and ITAM-containing proteins such as CD79a and CD79b\u003cbr\u003e\nCell Type: B cells, Leukemia\u003cbr\u003e\nBiology Area: Cell Biology, Immuno-Oncology, Immunology\u003cbr\u003e\nAntigen References: Dymecki SM, et al. 1990. Science. 247:332-6. Zhang H, et al. 2012. Nat Genet. 44:861-71. Malek SN, et al. 1998. Proc Natl Acad Sci USA. 95:7351-6. Akerblad P, et al. 1999. J Immunol. 163:5453-61.\u003cbr\u003e\nGene ID: 640\u003cbr\u003e\nUniProt: View information about BLK on UniProt.org\u003cbr\u003e\nClone: W21005C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tyrosine-protein kinase Blk, B lymphocyte kinase, p55-BLK\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866426167465,"sku":"642352","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-642352","provider":"Iright","version":"1.0","type":"link"}