{"product_id":"biolegend-643807","title":"Biolegend, 643807, Direct-Blot™ HRP anti-β-actin Antibody, 100microl","description":"\u003cp\u003eβ-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration\/dilution.\u003cbr\u003e\nApplication Notes: The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS\/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Lawson BR, et al. 2007. J. Immunol. 178:5366. Joyce CW, et al. 2006. J. Biol. Chem. 281:33053. Yanagiya T,et al.2007.Obesity.15:572. KishidaT,et al. 2007.J. Immunol. 179:8554. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144. Toltlt LJ,et al. 2008.J. Immunol. 181:2165. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820. Ikeda D, et al. 2008. Endocrinology. 149:6037. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed\u003cbr\u003e\nProduct Citations: Hochrein SM, et al. 2022. Cell Metab. 34:516. PubMed Eguiarte-Solomon F, et al. 2021. Mol Carcinog. 60:342. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Kyle Cottrell, Sergej Djuranovic 2016. PLoS One. 11:e0167877. PubMed Vassilieva EV, et al. 2020. Front Immunol. 2.504166667. PubMed Doi T, et al. 2022. Heliyon. 8:e08890. PubMed Zacks DN, et al. 2022. J Clin Med. 11:. PubMed Wang J, et al. 2021. Biomolecules. 11:. PubMed\u003cbr\u003e\nRRID: AB_2734515 (BioLegend Cat. No. 643808) AB_2566701 (BioLegend Cat. No. 643807)\u003cbr\u003e\nStructure: Actin, cytoplasmic 1\u003cbr\u003e\nDistribution: Ubiquitously expressed in the cytoplasm of all eukaryotic cells\u003cbr\u003e\nFunction: Actins are highly conserved proteins that are involved in cell motility\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Microfilaments\u003cbr\u003e\nAntigen References: 1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673. 2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133. 3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.\u003cbr\u003e\nGene ID: 11461\u003cbr\u003e\nUniProt: View information about beta-actin on UniProt.org\u003cbr\u003e\nClone: 2F1-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Actin, cytoplasmic 1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862322237609,"sku":"643807","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-643807","provider":"Iright","version":"1.0","type":"link"}