{"product_id":"biolegend-644805","title":"Biolegend, 644805, PerCP\/Cyanine5.5 anti-T-bet Antibody, 25μg","description":"\u003cp\u003eT-bet, also known as T-box transcription factor T-bet, is considered to be a \"master regulator\" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix\/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.\u003cbr\u003e\nProduct Citations: Read KA, et al. 2023. Nat Commun. 14:1652. PubMed Nunes-Santos CJ, et al. 2023. Nat Commun. 14:3708. PubMed Wang W, et al. 2022. Cell Rep. 41:111582. PubMed Glaubitz J, et al. 2023. Gut. :. PubMed Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed Zander R, et al. 2022. Immunity. 55:475. PubMed Frasca D, et al. 2019. PLoS One. 14:e0219545. PubMed Amelio P, et al. 2017. PLoS Negl Trop Dis. 10.1371\/journal.pntd.0005817. PubMed Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed Huanosta-Murillo E, et al. 2021. Front Immunol. 12:668369. PubMed Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Marro BS, et al. 2019. Cell Rep. 29:3293. PubMed Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed Webb ER, et al. 2022. iScience. 25:104995. PubMed Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed Hurton L, et al. 2016. Proc Natl Acad Sci U S A. 113: E7788 - E7797. PubMed Nabatanzi R, et al. 2021. BMC Immunol. 22:59. PubMed Minns D, et al. 2021. Nat Commun. 12:1285. PubMed Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed\u003cbr\u003e\nRRID: AB_1595593 (BioLegend Cat. No. 644805) AB_1595488 (BioLegend Cat. No. 644806)\u003cbr\u003e\nStructure: T-box transcription factor, approximately 58 kD.\u003cbr\u003e\nDistribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.\u003cbr\u003e\nFunction: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.\u003cbr\u003e\nCell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.\u003cbr\u003e\nGene ID: 30009\u003cbr\u003e\nUniProt: View information about T-bet on UniProt.org\u003cbr\u003e\nClone: 4B10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T-box expressed in T cells, T box 21, TBLYM\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862408482985,"sku":"644805","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-644805","provider":"Iright","version":"1.0","type":"link"}