{"product_id":"biolegend-645102","title":"Biolegend, 645102, Purified anti-Caspase-1 Antibody, 100μg","description":"\u003cp\u003eCaspase -1 plays a vital role in cleaving the inactive procytokine pro-IL-1 beta to generate the active 17 kd mature form of IL-1beta, a key mediator of inflammation. Active caspase-1 (ICE) is derived a single proenzyme of 45 Kd. An active ICE comprises two subunits of P20 and two subunits of P10 after removal a large amino-terminal pro-domain and a small linker peptide. Caspase-1 is reported to be involved in CD95- mediated apoptosis. Four alternatively spliced variants of caspase-1 have been reported, with mw ranging from 30-48 kd.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Bacterial fusion protein (p20)\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1-2µg antibody per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kang SJ, et al. 2000. J Cell Biol 149:613 Kang SJ, et al. 2002. Cell Death and Differentiation. 9:1115\u003cbr\u003e\nProduct Citations: Zhao YJ, et al. 2022. Neural Regen Res. 17:1324. PubMed Liu L, et al. 2017. J Crohns Colitis. 10.1093\/ecco-jcc\/jjw219. PubMed Zhu Q, et al. 2016. Am J Physiol Renal Physiol. 310: F621-F627. PubMed Reis AS, et al. 2020. Sci Adv. 6:eaax6346. PubMed Nastase MV, et al. 2016. The American Journal of Pathology. 186(5):1206-20. PubMed\u003cbr\u003e\nRRID: AB_2068901 (BioLegend Cat. No. 645101) AB_2068900 (BioLegend Cat. No. 645102)\u003cbr\u003e\nStructure: The whole length of Caspase-1 is a 404 amino acid polypeptide chain with MW of 45kd. The active Caspase -1 is a tetramer compariseing two subunits of 20Kd and 10Kd.\u003cbr\u003e\nDistribution: Predominantly in the cytoplasm, some localized to the external cell membrane.\u003cbr\u003e\nFunction: Caspase-1 is a mediator of the inflammatory response by cleaving the inactive pro-IL1 beta at Asp116\/Ala 117 to generate the active 17k mature form of IL-1 beta.\u003cbr\u003e\nLigand\/Receptor: Pro-IL1 beta\u003cbr\u003e\nBioactivity: IL-1beta converting enzyme (ICE)\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Neuroscience\u003cbr\u003e\nAntigen References: 1. Wilson KP et al. 1994. Nature 370:251 2. Cohen GM:1997 Biochem J 326:1\u003cbr\u003e\nGene ID: 834\u003cbr\u003e\nUniProt: View information about Caspase-1 on UniProt.org\u003cbr\u003e\nClone: 5B10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ICE\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862447411369,"sku":"645102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-645102","provider":"Iright","version":"1.0","type":"link"}