{"product_id":"biolegend-648452","title":"Biolegend, 648452, Purified anti-SDHA Antibody, 100μg","description":"\u003cp\u003eSuccinate dehydrogenase (SDH), commonly known as respiratory complex II, is an important enzyme of mitochondria in the citric acid cycle and electron transport chain, and consists of four subunits SDHA, SDHB, SDHC, and SDHD. SDHA, like other SDHx genes, can function as a tumor suppressor gene. Recently, studies have indicated that mutations in SDHA contribute to various disorders such as hereditary pheochromocytoma-paraganglioma (PPGL) syndrome, mitochondrial complex II deficiency, gastrointestinal stromal tumors, Leigh syndrome, dilated cardiomyopathy, and neuroendocrine tumors.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant human SDHA\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg\/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: This antibody has been tested in knockout\/knockdown systems for WB and ICC. This antibody (clone W23126E) does not cross-react with mouse SDHA on Western Blot (WB). For immunocytochemistry (ICC), we recommend fixation with 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation. For immunohistochemistry on FFPE tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 (Cat. No. 422704) is recommended.\u003cbr\u003e\nRRID: AB_3717114 (BioLegend Cat. No. 648451) AB_3717114 (BioLegend Cat. No. 648452)\u003cbr\u003e\nStructure: SDHA is a 664 amino acid protein with a predicted molecular weight of 73 kD.\u003cbr\u003e\nDistribution: Membrane and mitochondrion inner membrane\u003cbr\u003e\nFunction: An important enzyme of mitochondria in the citric acid cycle and electron transport chain, tumor suppressor gene, prognostic marker\u003cbr\u003e\nInteraction: Interacts with SDHAF2\/SDH5, TRAP1, LACC1.\u003cbr\u003e\nBiology Area: Cardiovascular Biology, Cell Biology, Cell Death, Cell Proliferation and Viability, Neurodegeneration, Neuroscience\u003cbr\u003e\nMolecular Family: Mitochondrial Markers, Tumor Suppressors\u003cbr\u003e\nAntigen References: Oyedotun KS, et al. 2004. J Biol Chem. 279: 9424. Rutter J, et al.2011. Mitochondrion.10:393. Burnichon N. 2010. Hum Mol Genet.19: 3011. Benchoua A, et al. 2006. Mol Biol Cell. 17:1652.\u003cbr\u003e\nGene ID: 6389\u003cbr\u003e\nUniProt: View information about SDHA on UniProt.org\u003cbr\u003e\nClone: W23126E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866438521001,"sku":"648452","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-648452","provider":"Iright","version":"1.0","type":"link"}