{"product_id":"biolegend-648879","title":"Biolegend, 648879, Purified Anti-Musashi-2 Antibody, 100μg","description":"\u003cp\u003eMusashi-2 (MSI2) is a highly conserved RNA-binding protein that plays a critical role in post-transcriptional regulation of gene expression, particularly in stem cell maintenance, differentiation, and proliferation. Musashi-2 is essential for normal hematopoiesis and neural development, where it regulates the translation of mRNAs involved in cell cycle and developmental pathways. Dysregulation has been implicated in a variety of cancers, such as acute myeloid leukemia (AML) and MLL-rearranged acute lymphoblastic leukemia (ALL), where MSI2 supports leukemic stem cell survival and contributes to therapy resistance. Musashi-2 also promotes tumor progression in solid malignancies like pancreatic cancer, by modulating pathways such as PI3K\/AKT\/mTOR and epithelial-mesenchymal transition (EMT). Elevated Musashi-2 expression is frequently associated with poor prognosis, increased metastasis, and resistance to conventional therapies. This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 WT Cell Line (Horizon Cat No. Cat. No. C631) and HAP1 MSI2 Knockout cell line (Horizon Cat. No. HZGHC006122c011)\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant fragment of human Musashi-2 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P, ICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg\/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg\/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401). Similar results were also obtained using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803). For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).\u003cbr\u003e\nRRID: AB_3720622 (BioLegend Cat. No. 648878) AB_3720622 (BioLegend Cat. No. 648879)\u003cbr\u003e\nStructure: Musashi-2 protein consists of 328 amino acids and has a molecular weight of approximately 35.2 kDa.\u003cbr\u003e\nDistribution: Cytoplasm\u003cbr\u003e\nInteraction: RHOBTB2, HNRNPH1 and HNRNPH2, MAPT (Tau protein), MEOX2, TIMM10B\u003cbr\u003e\nCell Type: Hematopoietic stem and progenitors, Leukemia, Monocytes, Neural Stem Cells, T cells\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, DNA Repair\/Replication\u003cbr\u003e\nAntigen References: Huang L, et al. 2024. Cancer Cell Int. 24(1):273. Jiang L, et al. 2022. Front Oncol. 12:969632. Zang H, et al. 2025. Leukemia. 39(1):265. Taggart J, et al. 2016. Nat Commun. 7:10739.\u003cbr\u003e\nGene ID: 124540\u003cbr\u003e\nUniProt: View information about Musashi-2 on UniProt.org\u003cbr\u003e\nClone: W23272C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MSI2, MSI2H, Musashi-2, Musashi RNA Binding Protein 2, RNA-Binding Protein Musashi Homolog 2\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866439700649,"sku":"648879","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-648879","provider":"Iright","version":"1.0","type":"link"}