{"product_id":"biolegend-650152","title":"Biolegend, 650152, Purified anti-human CD20 Antibody, 100μg","description":"\u003cp\u003eCD20 is a cell surface protein primarily expressed on B lymphocytes which plays a key role in the regulation of B cell activation, proliferation, and differentiation. Encoded by the gene MS4A1, homo-oligomeric complexes of CD20 form calcium conductive ion channels that modulate signaling pathways necessary for B cell survival and immune responses. CD20 plays a role in B cell activation of downstream Src family kinases such as Lyn, Lck, and Fyn. Because of its selective expression on B cells CD20 has become a target for therapeutic monoclonal antibodies to treat conditions like B cell lymphomas and autoimmune diseases.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant fragment of the N terminus of human CD20 (cytosolic)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2˚C and 8˚C.\u003cbr\u003e\nApplication: IHC-P - Quality tested ICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg\/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg\/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix\/permeabilize with either of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only For use in intracellular flow cytometry (ICFC), it is recommended to fix\/permeabilize with Cyto-Fast™ Fix\/Perm Buffer Set (Cat. No. 426803) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). This product is not recommended for use in Western Blot.\u003cbr\u003e\nRRID: AB_3699076 (BioLegend Cat. No. 650151) AB_3699076 (BioLegend Cat. No. 650152)\u003cbr\u003e\nStructure: CD20 is a 297 amino acid protein with a predicted molecular weight of 33.1 kD.\u003cbr\u003e\nDistribution: Cell membrane, B cells\u003cbr\u003e\nFunction: Calcium channel\u003cbr\u003e\nInteraction: MHC class I and II, CD53, CD81, CD82\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Morsy DE, et al. 2013. J Immunol. 191:3112. Lund FE and Randall TD. 2010. Nat Rev Immunol. 10:236. Beers SA, et al. 2010. Blood. 115:5191. Kuijpers TW, et al. 2010. J Clin Invest. 120:214.\u003cbr\u003e\nGene ID: 931\u003cbr\u003e\nUniProt: View information about CD20 on UniProt.org\u003cbr\u003e\nClone: W23056F\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Cluster of differentiation 20 (CD20), membrane-spanning 4-domains subfamily A member 1 (MS4A1), B1, Bp35\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866437275817,"sku":"650152","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-650152","provider":"Iright","version":"1.0","type":"link"}