{"product_id":"biolegend-652501","title":"Biolegend, 652501, Purified anti-His Tag Antibody, 25μg","description":"\u003cp\u003eThis antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Epitope tag\u003cbr\u003e\nReported Reactivity: Species independent\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: KLH-conjugated His-tag peptide\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml (Dilution 1:1000 - 1:5000). For immunocytochemistry, a concentration range of 0.1 - 0.5 μg\/ml (Dilution 1:1000 - 1:5000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Burritt JB, et al. 1995. J. Biol. Chem. 270:16974. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.\u003cbr\u003e\nProduct Citations: Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed Goosens VJ, et al. 2021. ACS Synth Biol. 10:3422. PubMed Lam B, et al. 2021. Cell Rep. 37:109838. PubMed Shigemori S, et al. 2017. J Dairy Sci. 10.3168\/jds.2017-12872. PubMed Mamedov T, et al. 2017. PLoS One.. 10.1371\/journal.pone.0183589. PubMed Namai F, et al. 2017. Curr Microbiol. 10.1007\/s00284-017-1354-3. PubMed Vunnam N, et al. 2020. Biochemistry. 59:3856. PubMed Zoulias N, et al. 2021. Curr Biol. S0960-9822:01407. PubMed Williford JM, et al. 2019. Sci Adv. 5:eaay1357. PubMed Liu L, et al. 2019. J Cell Biol. 218:644. PubMed Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed Mamedov T, et al. 2021. Front Plant Sci. 12:742875. PubMed Manrique-Suárez V, et al. 2021. Proteins. 89:1508. PubMed Yumoto K, et al. 2020. Antioxidants (Basel). 9:00. PubMed Keshwani M, et al. 2015. Biochem J. 472: 329 - 338. PubMed\u003cbr\u003e\nRRID: AB_11204080 (BioLegend Cat. No. 652501) AB_11204427 (BioLegend Cat. No. 652502) AB_2564271 (BioLegend Cat. No. 652505)\u003cbr\u003e\nFunction: Recombinant poly-histidine tag detection\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about His Tag on UniProt.org\u003cbr\u003e\nClone: J099B12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Histidine Tag\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866110415017,"sku":"652501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-652501","provider":"Iright","version":"1.0","type":"link"}