{"product_id":"biolegend-652601","title":"Biolegend, 652601, Purified anti-IRF5 Antibody, 25μg","description":"\u003cp\u003eIRF-5 is a transcription factor belonging to the interferon regulatory factor family. It controls induction of type I IFN genes as well as inflammatory cytokines and chemokines through TLRs and the MyD88-TRAF6 pathway. Several genetic variations of IRF-5 have been reported as a risk factor of Systemic lupus erythematosus (SLE), which is caused by excess IFNα induction.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human IRF-5 recombinant protein (176-240 aa).\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedIP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2-10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Internal testing suggests this clone is not suitable for intracellular flow cytometry.\u003cbr\u003e\nProduct Citations: Breuer J, et al. 2014. Ann Neurol. 739:75. PubMed\u003cbr\u003e\nRRID: AB_2561469 (BioLegend Cat. No. 652601) AB_2561470 (BioLegend Cat. No. 652602)\u003cbr\u003e\nStructure: 498 aa with predicted molecular weight of 56 kD. The protein contains a DNA binding domain (IRF tryptophan pentad repeat) and a poly-Glutamate region.\u003cbr\u003e\nDistribution: Shuttles between the nucleus and the cytoplasm.\u003cbr\u003e\nFunction: The transcription factor IRF-5 participates in the virus-mediated activation of interferon and regulates gene expression induced by cell growth, differentiation, and apoptosis.\u003cbr\u003e\nInteraction: IKKα phosphorylates IRF-5 and induces formation of IRF-5 dimers. TRAF6 mediates K63-linked ubiquitination of IRF-5, required for nuclear translocation of IRF-5 and regulation of target genes. IRF-5 assemblies with CBP\/p300 to initiate transcription.\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors\u003cbr\u003e\nAntigen References: 1. Balkhi MY, et al. 2010. Cell. Signal. 1:117. 2. Balkhi MY, et al. 2008. Mol. Cell. Biol. 28:7296. 3. Salloum R and Niewold TB. 2011. Transl. Res. 157:326. 4. Barnes BJ, et al. 2003. J. Biol. Chem. 278:16630. 5. Rullo OJ, et al. 2010. Ann. Rheum. Dis. 69:611.\u003cbr\u003e\nGene ID: 3663\u003cbr\u003e\nUniProt: View information about IRF5 on UniProt.org\u003cbr\u003e\nClone: 11F4A09\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interferon regulatory factor 5 (IRF-5)\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864325738665,"sku":"652601","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-652601","provider":"Iright","version":"1.0","type":"link"}