{"product_id":"biolegend-654702","title":"Biolegend, 654702, Purified anti-SWAP70 Antibody, 100μg","description":"\u003cp\u003eSWAP70 was initially isolated as a component of an isotype switch recombination complex (SWAP) from activated B lymphocytes. It translocates from cytoplasm to the nucleus upon B cell activation. SWAP70 interacts with F-actin and RAC, binds to PI(3,4)P 2 , and regulates membrane ruffling and lamellipodia formation in response to growth factor stimulation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human SWAP70 recombinant protein (89-585 aa).\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.\u003cbr\u003e\nPreparation: Affinity purified.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-0.5 µg per ml. For immunoprecipitation, the suggested use of this reagent is 10-30 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2561890 (BioLegend Cat. No. 654702)\u003cbr\u003e\nStructure: Contains a PH domain, which is required for binding of phosphatidylinositol 3,4,5-trisphosphate, a coil-coil domain, and a domain weakly homologous to DH domains. Theoretical molecular weight is 69 kD.\u003cbr\u003e\nDistribution: SWAP70 is localized to cytoplasm in resting B-cells, recruited to plasma membrane, and translocated to the nucleus upon B-cell activation.\u003cbr\u003e\nFunction: SWAP70 is a guanine nucleotide exchange factor (GEF) which binds phosphatidylinositol trisphosphate and transduces signals from tyrosine kinase receptors to RAC. SWAP70 is required for membrane ruffling and lamellipodia formation during cytoskeletal F-actin rearrangement.\u003cbr\u003e\nInteraction: Interacts with cytoskeletal F-actin and Rac to regulate actin remodeling. Associates with NPM1, NCL, and PARP1 in switch-associated protein complex.\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Immunology, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Masat L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:2180. 2. Hilpelä P, et al. 2003. Mol. Biol. Cell. 14:3242. 3. Ihara S, et al. 2006. J. Cell Sci. 119:500. 4. Shinohara M, et al. 2002. Nature 416:759. 5. Pearce G, et al. 2006. Nat. Immunol. 7:827.\u003cbr\u003e\nGene ID: 23075\u003cbr\u003e\nUniProt: View information about SWAP70 on UniProt.org\u003cbr\u003e\nClone: 9D9A19\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: SWAP switching B-cell complex 70 kD subunit, switch-associated protein 70\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864380559529,"sku":"654702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-654702","provider":"Iright","version":"1.0","type":"link"}