{"product_id":"biolegend-654804","title":"Biolegend, 654804, PE anti-BATF Antibody, 100tests","description":"\u003cp\u003eBATF is a basic leucine zipper transcription factor belonging to the ATF superfamily. BATF forms heterodimers with Jun proteins, which in turn binds to AP-1 consensus sites. The BATF\/Jun heterodimers have significantly reduced transcriptional activity compared to Fos\/Jun heterodimers. Therefore, induction of BATF expression suppresses AP-1 target genes by competing for Fos\/Jun binding sequences. BATF is expressed at low levels in hematopoietic cells and can be induced in response to a variety of stimuli. BATF knockout mice show defects in cytokine expression and in the differentiation of T helper cell subsets and B cells, but not in the proliferation of lymphocytes. These findings suggest that BATF is a tissue-specific regulator for AP-1 activity and plays an essential role in the regulation of immune responses. During early EBV infection, BATF expression is induced by EBNA2 proteins, subsequently preventing infected B cells from entering apoptotic and lytic pathways. This indicates that BATF functions as a modulator to maintain latency status of virus-infected cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full length human BATF recombinant protein.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nProduct Citations: Pham D, et al. 2019. Cell Rep. 29:1203. PubMed\u003cbr\u003e\nRRID: AB_2563516 (BioLegend Cat. No. 654803) AB_2563517 (BioLegend Cat. No. 654804)\u003cbr\u003e\nStructure: 14 kD protein containing a bZIP (basic-leucine zipper) domain, which mediates dimerization with Jun family members.\u003cbr\u003e\nDistribution: Nucleus. Expressed most abundantly in hematopoietic tissues.\u003cbr\u003e\nFunction: AP-1 transcription complexes regulate gene expression in response to various stimuli, such as cytokines, growth factors, stress, and infection. BATF belongs to the AP-1\/ATF superfamily and negatively regulates AP-1 mediated transcription when it forms heterodimers with Jun proteins.\u003cbr\u003e\nInteraction: Forms heterodimers with c-Jun, JunB, and JunD. Interacts with IFI35, IRF4, and IRF8 through bZIP domain.\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Betz BC, et al. 2010. J. Exp. Med. 207:933. 2. Dorsey MJ, et al. 1995. Oncogene 11:2255. 3. Echlin DR, et al. 2000. Oncogene 19:1752. 4. Johansen LM, et al. 2003. J. Virol. 77:6029. 5. Schraml BU, et al. 2009. Nature 460:405. 6. Tussiwand R, et al. 2012. Nature 490:502.\u003cbr\u003e\nGene ID: 10538\u003cbr\u003e\nUniProt: View information about BATF on UniProt.org\u003cbr\u003e\nClone: 9B5A13\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B-cell-activating transcription factor, Basic leucine zipper transcriptional factor ATF-like, SFA-2, BATF1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864380330153,"sku":"654804","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-654804","provider":"Iright","version":"1.0","type":"link"}