{"product_id":"biolegend-655702","title":"Biolegend, 655702, Purified anti-IRF3 Antibody, 100μg","description":"\u003cp\u003eIRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine\/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB protein binding to dsRNA-activated factor 1, a complex which activates the transcription of genes under control of ISRE. The phosphorylated IRF-3 is under negative regulation by ubiquitin-mediated degradation. Research indicates that it plays a critical role in stimulated TLR-mediated IL-27 p28 gene expression.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial IRF-3 recombinant protein (200-427 a.a.)\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: Affinity purified\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ChIP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Ta A, et al. 2023. Nat Commun. 14:2035. PubMed He X, et al. 2017. PLoS One. 13(6):e1006347. PubMed Kanno T, et al. 2022. Front Immunol. 13:904875. PubMed Raychowdhury R, et al. 2021. Immunohorizons. 5:818. PubMed\u003cbr\u003e\nRRID: AB_2562201 (BioLegend Cat. No. 655701) AB_2562202 (BioLegend Cat. No. 655702)\u003cbr\u003e\nStructure: Human IRF-3 has 427 amino acids with predicted Mw 47.2kD and observed Mw 52kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9kD and observed Mw 45kD.\u003cbr\u003e\nDistribution: Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine\/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.\u003cbr\u003e\nFunction: IRF-3 is a constitutively expressed, latent transcription factor that plays a pivotal role in type I IFN response.\u003cbr\u003e\nInteraction: CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Lin R, et al. 1999. Mol. Cell Biol. 19:959. 2. Hiscott J, et al. 2007. J. Biol. Chem. 282:15325. 3. Molle C, et al. 2010. J. Immunol. 184:1784.\u003cbr\u003e\nGene ID: 3661\u003cbr\u003e\nUniProt: View information about IRF3 on UniProt.org\u003cbr\u003e\nClone: 12A4A35\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interferon regulatory factor-3 (IRF-3)\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864326951081,"sku":"655702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-655702","provider":"Iright","version":"1.0","type":"link"}