{"product_id":"biolegend-656403","title":"Biolegend, 656403, PE anti-Th-POK (ZFP-67) Antibody, 25tests","description":"\u003cp\u003eTh-POK is a member of the BTB-POZ domain-containing zinc finger transcription factor family. It regulates gene expression during intrathymic T cell differentiation. A single autosomal recessive mutation of the Th-POK gene results in the absence of mature CD4+ helper T cells, called helper-deficient (HD) phenotype. In Th-POK deficient mice, MHC class II restricted thymocytes are redirected to the CD8+ T cell lineage. On the contrary, forced expression of Th-POK redirects MHC class I restricted thymocytes to the CD4+ T cell lineage. Inactivation of Th-POK in mature CD4+ T cells results in induction of the genes preferentially expressed in CD8+ T cells, such as CD8, granzyme B, and IFN-γ. These findings suggest that Th-POK is essential in promoting commitment of immature T cells to the CD4 lineage and maintaining the characteristics of mature CD4+ T helper cells.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human Th-POK recombinant protein (197-345 a.a.)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended. This clone displays a similar affinity to BioLegend clone W15147A by western blot. However, this clone does not recognize mouse TH-POK.\u003cbr\u003e\nRRID: AB_2563008 (BioLegend Cat. No. 656403) AB_2563009 (BioLegend Cat. No. 656404)\u003cbr\u003e\nStructure: 539 amino acids, predicted molecular weight of 58 kD; contains four C2H2-type zinc finger regions that are responsible for DNA binding; contains a BTB-POZ domain which is involved in homodimerization and association with other factors\u003cbr\u003e\nDistribution: Nucleus\u003cbr\u003e\nFunction: Transcription factor that regulates CD4 lineage commitment of immature T-cell precursors; acts as a transcriptional repressor of collagen and fibronectin genes\u003cbr\u003e\nInteraction: Acetyltransferase p300\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Kappes DJ. 2010. Immunol. Rev. 238:182. 2. Mariani F, et al. 2013. PLoS One. 8:e54488. 3. Zhang M, et al. 2010. J. Immunol. 185:3960. 4. Egawa T, et al. 2008. Nat. Immunol. 9:1131. 5. Wildt KF, et al. 2007. J. Immunol. 179:4405. 6. Setoguchi R, et al. 2009. J. Immunol. 183:4467.\u003cbr\u003e\nGene ID: 51043\u003cbr\u003e\nUniProt: View information about Th-POK on UniProt.org\u003cbr\u003e\nClone: 11H11A14\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T-helper-inducing POZ\/Krueppel-like factor, Zinc finger and BTB domain-containing protein 7B (ZBTB7B), Krueppel-related zinc finger protein (cKrox), Zinc finger protein 67 homolog (ZFP-67)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864326754473,"sku":"656403","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-656403","provider":"Iright","version":"1.0","type":"link"}