{"product_id":"biolegend-656802","title":"Biolegend, 656802, Purified anti-Nestin Antibody, 100μg","description":"\u003cp\u003eNestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Fusion protein\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: For immunohistochemistry use on formalin-fixed paraffin-embedded tissue (IHC-P), antigen retrieval with Citrate Buffer, 10X (Cat. No. 420902) is recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)\u003cbr\u003e\nProduct Citations: Salgado B, et al. 2023. Microorganisms. 11:. PubMed Di Stefano B, et al. 2019. Cell Stem Cell. 25:622. PubMed Hu YB, et al. 2021. Aging Cell. 20:e13454. PubMed Feng H, et al. 2021. Stem Cell Research. 53:102346. PubMed Zelenova EA, et al. 2021. Cells. 10:. PubMed\u003cbr\u003e\nRRID: AB_2562473 (BioLegend Cat. No. 656801) AB_2562474 (BioLegend Cat. No. 656802)\u003cbr\u003e\nStructure: 1621 amino acids with predicted molecular weight of 177 kD. The observed molecular weight is larger than 250 kD.\u003cbr\u003e\nDistribution: Cytosol\u003cbr\u003e\nFunction: Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation\u003cbr\u003e\nInteraction: Vimentin, alpha-internexin and FHOD3\u003cbr\u003e\nCell Type: Neural Stem Cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: Intermediate Filaments\u003cbr\u003e\nAntigen References: Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59. Krupkova O Jr, et al. 2010. Neoplasma. 57:291. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.\u003cbr\u003e\nGene ID: 10763\u003cbr\u003e\nUniProt: View information about Nestin on UniProt.org\u003cbr\u003e\nClone: 10C2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NES\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860778569897,"sku":"656802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-656802","provider":"Iright","version":"1.0","type":"link"}