{"product_id":"biolegend-656951","title":"Biolegend, 656951, Purified anti-TMEM97 Antibody, 25μg","description":"\u003cp\u003eSigma intracellular receptor 2 (σ2R), also known as SIGMAR2 or TMEM97, is a transmembrane protein that is associated with the endoplasmic reticulum (ER), membranes and lysosomes. It is widely distributed in normal tissues such as the brain, lungs, heart, skeletal muscles, testis, and ovary. TMEM97 has been demonstrated to regulate the cholesterol transporter NPC1 in maintaining cholesterol homeostasis. Elevated expression of TMEM97 is a potential prognostic biomarker for breast, lung, colon, ovarian, bladder and oral squamous cancers. TMEM97 is also implicated in several neurological disorders and age-related diseases such as Alzheimer's disease.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant human TMEM97 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For use in immunohistochemistry on FFPE tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) is recommended. This antibody (clone W22180E) does not cross-react with mouse TMEM97 in Western Blot. We do not recommend this antibody for use in immunocytochemistry (ICC).\u003cbr\u003e\nRRID: AB_3717126 (BioLegend Cat. No. 656951) AB_3717126 (BioLegend Cat. No. 656952)\u003cbr\u003e\nStructure: TMEM97 is a 176 amino acid protein with a predicted molecular weight of 21 kD.\u003cbr\u003e\nDistribution: Widely expressed in normal tissues with increased expression in proliferating cancer cells.\u003cbr\u003e\nFunction: Controlling cellular cholesterol levels, cancer biomarker, modulates the cytosolic Ca2+ concentration, dopaminergic transmission, and cocaine-induced addiction behavior.\u003cbr\u003e\nInteraction: Interacts with NPC1, PGRMC1, LDLR, histatin 1\/HTN1 and TSPO.\u003cbr\u003e\nCell Type: Microglia, Neurons\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroscience\u003cbr\u003e\nMolecular Family: GPCR\u003cbr\u003e\nAntigen References: Bartz, F. et al. 2009. Cell Metab. 10:63. Narayanan S, et al. 2011. Future Med Chem. 3:79. Oyer HM, et al. 2019. Front Pharmacol. 10:1141. Yi B, et al. 2017. J Neurochem. 140:561. Vazquez-Rosa E, et al. 2019. ACS Chem Neurosci.10:1595. Riad, A. et al. 2020. Mol Neurobiol. 57:3803.\u003cbr\u003e\nGene ID: 27346\u003cbr\u003e\nUniProt: View information about TMEM97 on UniProt.org\u003cbr\u003e\nClone: W22180E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MAC30, Meningioma-associated protein 30; σ2R; Sigma intracellular receptor 2, Sigma-2 receptor, Transmembrane protein 97, S2R\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866435080361,"sku":"656951","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-656951","provider":"Iright","version":"1.0","type":"link"}