{"product_id":"biolegend-657602","title":"Biolegend, 657602, Purified anti-IRF1 Antibody, 100μg","description":"\u003cp\u003eIRF1 is the first identified member of the interferon regulatory transcription factor family. Its expression is induced in response to pathogen infection or the stimulation of several cytokines, including type I and II IFN, IL-1, IL-6, and TNF-α. Upon stimulation, IRF1 is transcriptionally activated by STAT1 and NFκB transcription factors via the GAS (interferon-gamma-activated sequence) element in the IRF1 promoter. IRF1 plays important  roles in mediating immune response during infection and development of T cells and NK cells. In addition to the immunomodulatory functions, IRF1 has also shown to be involved in the regulation of cell cycle progression, tumor suppression, and apoptosis. IRF1-mediated transcription can be repressed by IRF2 through competing for the same binding region on the promoters.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human IRF1 recombinant protein (147-325 a.a.)\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: Affinity purified.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted at 4°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 μg per mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Velásquez LN, et al. 2017. J Leukoc Biol. 101:759. PubMed Bailey KA, et al. 2019. FASEB J. 33:12888. PubMed\u003cbr\u003e\nRRID: AB_2562631 (BioLegend Cat. No. 657602)\u003cbr\u003e\nStructure: 325 amino acids, predicted Mw is 37 kD and observed MW is 45kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding.\u003cbr\u003e\nDistribution: Nucleus.\u003cbr\u003e\nFunction: IRF1 is a transcription factor, regulating expression of IFN and IFN-inducible genes. It is also involved in regulating cell proliferation, apoptosis, tumor suppression, and various immune responses.\u003cbr\u003e\nInteraction: Forms homodimers. Interacts with p300, MyD88, and PIAS3.\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Cycle\/DNA Replication, Immunology, Innate Immunity, Transcription Factors\u003cbr\u003e\nAntigen References: 1. Yang W, et al. 2012. PLoS One 7:e50932. 2. Schwartz JL, et al. 2011. Int. J. Breast Cancer. 2011:912102. 3. Horiuchi M, et al. 2011. J. Neuroinflammation. 8:8. 4. Frontini M, et al. 2009. Nucleic Acids Res. 37:1073. 5. Yarilina A, et al. 2008. Nat. Immunol. 9:378. 6. Kano S, et al. 2008. Nat. Immunol. 9:34.\u003cbr\u003e\nGene ID: 3659\u003cbr\u003e\nUniProt: View information about IRF1 on UniProt.org\u003cbr\u003e\nClone: 13H3A44\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interferon regulatory factor 1 (IRF-1)\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864329474217,"sku":"657602","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-657602","provider":"Iright","version":"1.0","type":"link"}