{"product_id":"biolegend-659002","title":"Biolegend, 659002, Purified anti-TdT Antibody, 100μg","description":"\u003cp\u003eTerminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. TdT is a specialized DNA polymerase expressed early in pre-B and pre-T lymphoid cell development, and in acute lymphoblastic leukemia\/lymphoma cells. TdT contributes to the generation of junctional diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human TdT recombinant protein (1-508aa) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The 7H9A33 clone has been shown to be useful for western blotting of human and mouse TdT.\u003cbr\u003e\nRRID: AB_2562962 (BioLegend Cat. No. 659002)\u003cbr\u003e\nStructure: 510 amino acids, predicted molecular weight of 58 kD.\u003cbr\u003e\nDistribution: Nucleus.\u003cbr\u003e\nFunction: Template-independent DNA polymerase catalyzing the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. It is also involved in the addition of nucleotides at the N region junction of rearranged immunoglobulin heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.\u003cbr\u003e\nInteraction: Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone.\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Cell Biology, DNA Repair\/Replication, Immunology\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: 1. Yunk L, et al. 2009. J. Immunol. 183:452. 2. Kedzierska K, et al. 2008. J. Immunol. 181:2556. 3. Fuller SA, et al. 1985. Biochem. J. 231:105. 4. Feeney AJ, et al. 2001. J. Immunol. 167:3486. 5. Komori T, et al. 1993. Science 261:1171.\u003cbr\u003e\nGene ID: 1791\u003cbr\u003e\nUniProt: View information about TdT on UniProt.org\u003cbr\u003e\nClone: 7H9A33\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: DNA nucleotidylexotransferase (DNTT), Terminal addition enzyme, Terminal transferase\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864380264617,"sku":"659002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-659002","provider":"Iright","version":"1.0","type":"link"}