{"product_id":"biolegend-661302","title":"Biolegend, 661302, Purified anti-DDX5 Antibody, 100μg","description":"\u003cp\u003eDDX5, also named RNA helicase p68, is a prototypic member of the DEAD (Asp-Glu-Ala-Asp) box family. DDX5 has been shown to be involved in the processes requiring the alteration of RNA structures, such as the maturation of pre-mRNA, rRNA, and miRNA. DDX5 also plays important helicase-independent roles in transcriptional regulation. DDX5 can act as a transcriptional coactivator of the androgen receptor (AR), estrogen receptor α (ERα), tumor suppressor p53, MyoD, RUNX2, and β-catenin, which are involved in the regulation of cell proliferation, differentiation, and tumorigenesis. DDX5 has been shown to be up-regulated in various cancers, including breast cancer, prostate cancer, and colorectal cancer, suggesting that DDX5 may participate in tumor development. DDX5 can also act as a transcriptional repressor through interacting with HDAC1.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human DDX5 recombinant protein (482-614 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunocytochemistry, the suggested use of this reagent is 2.0 - 10 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2563942 (BioLegend Cat. No. 661302)\u003cbr\u003e\nStructure: 614 amino acids, predicted molecular weight of 69 kD. Contains a DEAD box domain, a helicase domain, and a transactivation domain.\u003cbr\u003e\nDistribution: Nucleus.\u003cbr\u003e\nFunction: DDX5 is an ATP-dependent RNA helicase, regulating RNA processing and ribosome biogenesis. DDX5 is also involved in transcription by acting as a transcriptional coactivator or a repressor.\u003cbr\u003e\nInteraction: DDX5 interacts with DDX17, RBM4, AGO1, AGO2, ESR1, p300, RUNX2, and HDAC1.\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Cycle\/DNA Replication, Transcription Factors\u003cbr\u003e\nAntigen References: 1. Iyer RS, et al. 2014. Cell Cycle. 13:1413. 2. Samaan S, et al. 2014. Nucleic Acids Res. 42:2197. 3. Fuller-Pace FV. 2013. Biochim. Biophys. Acta. 1829:756. 4. Wang D, et al. 2012. Mol. Cell Proteomics. 11:M111.011932. 5. Saporita AJ, et al. 2011. Cancer Res. 71:6708. 6. Clark EL, et al. 2008. Cancer Res. 68:7938.\u003cbr\u003e\nGene ID: 1655\u003cbr\u003e\nUniProt: View information about DDX5 on UniProt.org\u003cbr\u003e\nClone: 14B9A58\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864331440297,"sku":"661302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-661302","provider":"Iright","version":"1.0","type":"link"}