{"product_id":"biolegend-663903","title":"Biolegend, 663903, Anti-, 100microl","description":"\u003cp\u003eRNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: E. coli\u003cbr\u003e\nReported Reactivity: Other species\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Purified recombinant E. coli RNA polymerase β subunit.\u003cbr\u003e\nPreparation: Dried ascites\u003cbr\u003e\nStorage \u0026amp; Handling: Store lyophilized antibody at -20°C. Avoid repeat freeze\/thaw cycles.\u003cbr\u003e\nApplication: WB - Quality tested ELISA - Verified IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 µl of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconsitution. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription. Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)\u003cbr\u003e\nProduct Citations: Gibson JA, et al. 2022. Proc Natl Acad Sci U S A. 119:e2210115119. PubMed Heinrich K, et al. 2019. MBio. 10. PubMed Ju X, et al. 2019. Nat Microbiol. 1.490972222. PubMed Broder U, Jaeger T, Jenal U 2016. Nat Microbiol. 2:16184. PubMed Ting SY, et al. 2020. Cell Host Microbe. 28(2):313-321.e6. PubMed Ting SY, et al. 2022. Elife. 11:. PubMed Stringer AM, et al. 2020. Elife. 9:00. PubMed Icke C, et al. 2021. eLife. 10:00. PubMed Kouba T, et al. 2020. Nat Commun. 11:6419. PubMed Osuna BA, et al. 2020. Cell Host Microbe. 28:31. PubMed Pribis JP et al. 2019. Mol Cell. 74(4):785-800 . PubMed\u003cbr\u003e\nRRID: AB_2564524 (BioLegend Cat. No. 663903)\u003cbr\u003e\nStructure: 1252 amino acids with a predicted molecular weight of approximately 150 kD.\u003cbr\u003e\nDistribution: Bacteria.\u003cbr\u003e\nFunction: Transcriptional regulator in bacteria.\u003cbr\u003e\nBiology Area: Cell Biology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Thompson NE, et al. 1992. Biochemistry 31:7003. 2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477. 3. Hirata A, et al. 2008. Nature. 451:851. 4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155. 5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.\u003cbr\u003e\nGene ID: 948488\u003cbr\u003e\nUniProt: View information about RNA Polymerase beta on UniProt.org\u003cbr\u003e\nClone: 8RB13\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta\u003cbr\u003e\nPreviously: NeoClone Catalog# W0023\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862496465065,"sku":"663903","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-663903","provider":"Iright","version":"1.0","type":"link"}