{"product_id":"biolegend-666402","title":"Biolegend, 666402, Purified anti-STAT1 Phospho (Tyr701) Antibody, 100μg","description":"\u003cp\u003eSTAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated at Tyrosine 701 (Tyr701) by receptor-associated kinases, including JAK1, JAK2, and TYK2. This results in STAT1 dimerization and subsequent translocation to the nucleus, where it functions as a transcriptional activator. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: WB - Quality tested ICFC, ICC, ChIP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml (1:250-5000 dilution). For intracellular flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunocytochemistry, a range of 5.0 μg\/ml is recommended. For ChIP applications, the suggested dilution is 1:50 by volume. It is recommended that the reagent be titrated for optimal performance for each application. For intracellular flow cytometry our True-Nuclear™ Transcription Factor Staining Protocol is recommended.\u003cbr\u003e\nApplication Notes: Clone A17012A recognizes STAT1 phosphorylated at Tyrosine 701 (Tyr701). When using this clone for ICC, we recommend using methanol to permeabilize fixed cells.\u003cbr\u003e\nRRID: AB_2728499 (BioLegend Cat. No. 666401) AB_2728500 (BioLegend Cat. No. 666402)\u003cbr\u003e\nStructure: Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.\u003cbr\u003e\nDistribution: Ubiquitous tissue expression, nucleoplasmic-cytosolic distribution\u003cbr\u003e\nFunction: Immune response activation, cell signaling\u003cbr\u003e\nInteraction: STAT1, STAT2, IRF9, ERBB4, JAK1, JAK2, TYK2, TNK1, SHP2\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nMolecular Family: Nuclear Markers, Phospho-Proteins\u003cbr\u003e\nAntigen References: Moretti S, et al. 2017. J. Biol. Chem. 292: 1785. Wei J, et al. 2015. J. Immunol. 195: 2870. Sung PS, et al. 2015. Proc. Natl. Acad. Sci. USA. 112: 10443 Ooi EL, et al. 2014. Proc. Natl. Acad. Sci. USA. 111: 1909. Wu TR, et al. 2002. J. Biol. Chem. 277: 47572. Horvath, et al. 1996. J. Virol. 70: 647. Haque SJ, et al. 1995. J. Biol. Chem. 270: 25709.\u003cbr\u003e\nGene ID: 6772\u003cbr\u003e\nUniProt: View information about STAT1 Phospho Tyr701 on UniProt.org\u003cbr\u003e\nClone: A17012A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Signal Transduced Activator of Transcription 1, Transcription Factor ISGF-3 components p91\/p84\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864403857577,"sku":"666402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-666402","provider":"Iright","version":"1.0","type":"link"}