{"product_id":"biolegend-669601","title":"Biolegend, 669601, Purified anti-METTL14 Antibody, 25μg","description":"\u003cp\u003eMETTL14 forms a heterodimer with METTL3 called the m6A-methyltransferase complex (MTC) to methylate adenosine residues at the N6 position of eukaryotic mRNAs. MTC-induced methylation takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs. In the MTC complex, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate. Once methylated, the mRNAs are recognized by distinct proteins known as “readers” that will affect their stability, splicing, and\/or translation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial-length recombinant human METTL14 protein between 1-300 a.a.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedIP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: While this clone is able to immunoprecipitate METTL14 from whole cell extracts, the resulting fraction was not significantly enriched in METTL14; therefore, this clone may not be suitable for detecting transient or low-abundance complexes.\u003cbr\u003e\nProduct Citations: Liu T, et al. 2022. Cancer Sci. 113:446. PubMed\u003cbr\u003e\nRRID: AB_2814512 (BioLegend Cat. No. 669601) AB_2814513 (BioLegend Cat. No. 669602)\u003cbr\u003e\nStructure: METTL14 is a 456 amino acid protein with a molecular weight of 65 kD.\u003cbr\u003e\nDistribution: Localized to the mitochondria; ubiquitously expressed.\u003cbr\u003e\nFunction: Methylation; mRNA splicing; mRNA translation\u003cbr\u003e\nInteraction: METTL3\u003cbr\u003e\nBiology Area: Protein Synthesis\u003cbr\u003e\nAntigen References: Liu J, et al. 2014. Nat. Chem. Biol. 10.1038\/nchembio.1432. Nagase T, et al. 2000. DNA Res. 7:273-81. Yue Y, et al. 2018. Cell Discov. 4:10. Schöller E, et al. 2018. RNA. 10.1261\/rna.064063.117. Wang X, et al. 2016. Nature. 10.1038\/nature18298.\u003cbr\u003e\nGene ID: 57721\u003cbr\u003e\nUniProt: View information about METTL14 on UniProt.org\u003cbr\u003e\nClone: W18219A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Methyltransferase Like 14, N6-Adenosine-Methyltransferase Non-Catalytic Subunit, HMETTL14, KIAA1627\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866336481449,"sku":"669601","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-669601","provider":"Iright","version":"1.0","type":"link"}