{"product_id":"biolegend-673151","title":"Biolegend, 673151, Purified anti-STK4 Antibody, 25μg","description":"\u003cp\u003eSerine\/Threonine Kinase 4, also known as STK4 or MST1, is essential for regulating cellular processes like stress response, apoptosis, and organ size control. Functionally, STK4 is a stress-activated kinase that can induce apoptosis by entering the nucleus and causing chromatin condensation and DNA fragmentation. It plays a crucial role in the Hippo signaling pathway, which restricts cell proliferation and promotes apoptosis, thereby preventing tumor growth. STK4 is regulated through its interactions with other proteins in a kinase cascade, including STK3\/MST2 and LATS1\/2, which further modulate proteins involved in cell growth and apoptosis. Mutations or deficiencies in STK4 can lead to severe immunodeficiencies and other health issues.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant fragment of human STK4\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: For use of this antibody on immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend using Tris-EDTA buffer (Cat. No. 422704) for antigen retrieval. We do not recommend citrate buffer.\u003cbr\u003e\nRRID: AB_3683494 (BioLegend Cat. No. 673151) AB_3683494 (BioLegend Cat. No. 673152)\u003cbr\u003e\nStructure: STK4 is a 487 amino acid protein with a molecular weight of approximately 55 kDa.\u003cbr\u003e\nDistribution: The STK4 protein is primarily localized in the cytoplasm but upon activation can be found in the nucleus\u003cbr\u003e\nFunction: The regulation of STK4 involves several mechanisms: - Phosphorylation: STK4 is activated through phosphorylation - Protein Interactions: STK4 interacts with various proteins in the Hippo signaling pathway, including STK3\/MST2 and LATS1\/2 - Stress Response: STK4 is activated in response to cellular stress - Negative Regulation: STK4 can negatively regulate certain pathways, such as the TBK1-IRF3 signaling pathway\u003cbr\u003e\nInteraction: STK4 interacts with various proteins in the Hippo signaling pathway, including STK3\/MST2 and LATS1\/2.\u003cbr\u003e\nAntigen References: Abdollahpour H, et al. 2012. Blood. 119:3450. Jorgensen SE, et al. 2020. J Clin Immunology. 41:109. Guennoun A, et al. 2021. J Clin Immunology. 41:1839. Nehme NT, et al. 2012. Blood. 119:3458.\u003cbr\u003e\nGene ID: 6789\u003cbr\u003e\nUniProt: View information about STK4 on UniProt.org\u003cbr\u003e\nClone: W21145C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MST1, KRS2, YSK3, MAmmalian STE20-Like protein kinase 1, Serine\/Threonine-Protein Kinase Krs-2, Kinase responsive to stress 2\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866430853289,"sku":"673151","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-673151","provider":"Iright","version":"1.0","type":"link"}