{"product_id":"biolegend-675202","title":"Biolegend, 675202, Purified anti-NLRP12 Antibody, 100μg","description":"\u003cp\u003eNLRP12, a member of the Nod-like receptor (NLR) family of proteins, was originally suggested to act as an inflammasome forming component and a modulator of NF-κB signaling. Recent studies revealed that NLRP12 plays anti-inflammatory roles, as NLRP12-deficient mice are more susceptible to chemically induced colitis, and show a more severe infiltration of granulocytes and T cells. The colitis induced in NLRP12 -\/- mice is associated with increased production of proinflammatory cytokines and activation of both NF-κB and ERK signaling pathways. NLRP12 was reported to attenuate canonical NF-κB signaling by inhibiting IRAK-1 phosphorylation. NLRP12 also down-regulates non-canonical NF-κB signaling through interaction with TRAF3 and the NF-κB inducing kinase NIK.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human NLRP12 recombinant protein (93-1061 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2565493 (BioLegend Cat. No. 675202)\u003cbr\u003e\nStructure: 1061 amino acids with a predicted molecular weight of 120 kD. Contains a DAPIN domain, a NACHT-associated domain, and eight leucine-rich repeats (LRR).\u003cbr\u003e\nDistribution: Cytoplasm.\u003cbr\u003e\nFunction: NLRP12 negatively regulatres inflammation through attenuating both canonical and non-canonical NF-κB signaling.\u003cbr\u003e\nInteraction: Interacts with ASC and FAF1 through its DAPIN domain.\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Allen IC. 2014. Front Immunol. 5:169.2. Zaki MH, et al. 2014. Proc. Natl. Acad. Sci. USA 111:385.3. Chen GY. 2014. Eur. J. Immunol. 44:321.4. Vladimer GI. 2013. Curr. Opin. Microbiol. 16:23.5. Allen IC, et al. 2012. Immunity 36:742.6. Vladimer GI, et al. 2012. Immunity 37:96.\u003cbr\u003e\nGene ID: 91662\u003cbr\u003e\nUniProt: View information about NLRP12 on UniProt.org\u003cbr\u003e\nClone: 3D11A53\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NACHT, LRR and PYD domains-containing protein 12, Monarch-1, PYRIN-containing APAF1-like protein 7 (PYPAF7), Regulated by nitric oxide (RNO)\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864371646633,"sku":"675202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-675202","provider":"Iright","version":"1.0","type":"link"}