{"product_id":"biolegend-677703","title":"Biolegend, 677703, Direct-Blot™ HRP anti-mCherry Antibody, 100microl","description":"\u003cp\u003emCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. It is the most widely used photostable protein in the class of red-shifted fluorescent proteins 1,2 . mCherry derives from proteins initially isolated from Cnidarians .\u003cbr\u003e\n100microl\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: mCherry.\u003cbr\u003e\nFormulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration\/dilution.\u003cbr\u003e\nProduct Citations: Irie F, et al. 2021. J Biol Chem. 296:100481. PubMed\u003cbr\u003e\nRRID: AB_2566703 (BioLegend Cat. No. 677703)\u003cbr\u003e\nStructure: 236 amino acids with a predicted molecular weight of approximately 27 kD.\u003cbr\u003e\nFunction: Fluorescent protein.\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nAntigen References: 1. Shaner NC, et al. 2008. Nat. Methods 5:545. 2. Shaner NC, et al. 2005. Nat. Methods 2:905.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about mCherry on UniProt.org\u003cbr\u003e\nClone: 8C5.5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: DSRED\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862493515945,"sku":"677703","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-677703","provider":"Iright","version":"1.0","type":"link"}