{"product_id":"biolegend-680603","title":"Biolegend, 680603, Direct-Blot™ HRP anti-V5-tag Antibody, 100microl","description":"\u003cp\u003eEpitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: Epitope tag\u003cbr\u003e\nReported Reactivity: Species independent\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration\/dilution.\u003cbr\u003e\nRRID: AB_2734532 (BioLegend Cat. No. 680604) AB_2572008 (BioLegend Cat. No. 680603)\u003cbr\u003e\nStructure: 14 amino acid peptide (GKPIPNPLLGLDST)\u003cbr\u003e\nFunction: The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nAntigen References: 1. Uhlen M, et al. 1990. Meth Enzymol. 185:129. 2. Hanke T, et al. 1995. J. Virol. Methods 53:149. 3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551. 4. Thomas SM, et al. 1988. Cell 54:891.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about V5-tag on UniProt.org\u003cbr\u003e\nClone: 7\/4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: GKPIPNPLLGLDST epitope tag\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862483259561,"sku":"680603","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-680603","provider":"Iright","version":"1.0","type":"link"}