{"product_id":"biolegend-681202","title":"Biolegend, 681202, Purified anti-NR5A2 Antibody, 100μg","description":"\u003cp\u003eThe nuclear receptor subfamily 5 group A member 2, also known as NR5A2, is a 54 kD nuclear receptor protein. NR5A2 is a member of the FTZ-F1 sub-family and is highly expressed in the pancreas and to a lesser extent in the liver. NR5A2 contains a DNA binding domain, a hormone ligand-binding domain, and two zinc finger domains. It also binds to DNA as a monomer. NR5A2 is known to regulate the transcription of the liver enzyme cholesterol 7-alpha-hydrolase, which converts cholesterol to bile acids and also activates the hepatitis B virus enhancer II to contribute to the liver-specific expression of this virus. NR5A2 has been suggested to regulate pancreas-specific gene expression and to play a role in embryonic development. There are at least 3 alternatively spliced isoforms of NR5A2: isoforms 1 and 2 are present in fetal and adult liver and in liver cancer cells HepG2 as well.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human NR5A2 recombinant protein (1-200 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2566455 (BioLegend Cat. No. 681202)\u003cbr\u003e\nStructure: Orphan nuclear receptor, member of the FTZ-F1 sub-family, contains a DNA binding domain, 2 zinc finger motifs, a FTZ-F1 box, and a ligand-binding domain. Approximately 54 kD, three alternatively spliced variants exist.\u003cbr\u003e\nDistribution: Nuclear, highly expressed in the pancreas, lower expression in the liver, and very low expression in the heart and lungs.\u003cbr\u003e\nFunction: Binds DNA as a monomer to regulate the transcription of the liver enzyme cholesterol 7-alpha-hydroxylase, which converts cholesterol to bile acids. Activates viral hepatitis B enhancer II to regulate liver-specific expression of this virus in combination with other transcription factors.\u003cbr\u003e\nInteraction: Interacts wtih GRIP1, NCOA2, and NR0B2.\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Li M, et al. 1998. J. Biol. Chem. 273:29022.2. Nitta M, et al. 1999. Proc. Natl. Acad. Sci. USA 96:6660.3. Lee YK, et al. 2002. J. Biol. Chem. 277:2463.4. Zhang CK, et al. 2001. Gene 273:239.\u003cbr\u003e\nGene ID: 2494\u003cbr\u003e\nUniProt: View information about NR5A2 on UniProt.org\u003cbr\u003e\nClone: 1D12B67\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Nuclear receptor subfamily 5 group A member 2, orphan nuclear receptor NR5A2, HB1F, fetoprotein transcription factor, FTF, CPF, CYP7A promoter binding factor, LRH1,B1F2, FTZ-F1, hB1F-2, FTZ-F1beta\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864361881769,"sku":"681202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-681202","provider":"Iright","version":"1.0","type":"link"}