{"product_id":"biolegend-681301","title":"Biolegend, 681301, Purified anti-SPI1 (PU.1) Antibody, 25μg","description":"\u003cp\u003eSPI1 is a transcription factor that belongs to the E26-transformation-specific (ETS) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during the differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as malignancy. The overexpression of SPI1 blocks erythroid differentiation and inhibits cell death. Mice carrying a mutant SPI1 allele shows decreased SPI1 expression and develops acute myeloid leukaemia (AML), which suggests that SPI1 plays a role in oncogenesis.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Full length SPI1 recombinant protein expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 10 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2566468 (BioLegend Cat. No. 681301) AB_2566518 (BioLegend Cat. No. 681302)\u003cbr\u003e\nStructure: 272 amino acids with a predicted molecular weight of 31.3 kD. It contains a C-terminal ETS domain that is responsible for DNA binding.\u003cbr\u003e\nDistribution: Nucleus.\u003cbr\u003e\nFunction: SPI1 is a transcription factor that is required for the development of lymphoid and myeloid cells.\u003cbr\u003e\nInteraction: SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Hikami K, et al. 2011. Arthritis Rheum. 63:755. 2. Zakrzewska A, et al. 2010. Blood 116:e1. 3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391. 4. Pospisil V, et al. 2011. EMBO J. 30:4450. 5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922. 6. Rimmele P, et al. 2010. Cancer Res. 70:6757.\u003cbr\u003e\nGene ID: 20375\u003cbr\u003e\nUniProt: View information about SPI1 on UniProt.org\u003cbr\u003e\nClone: 7C2C34\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Spleen focus forming virus (SFFV) proviral integration oncogene, Sfpi1, Transcription factor PU.1, Dis1, PU.1, Dis-1, Sfpi1, Spi-1, Sfpi-1, Tcfpu1, Tfpu.1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864377413801,"sku":"681301","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-681301","provider":"Iright","version":"1.0","type":"link"}