{"product_id":"biolegend-681702","title":"Biolegend, 681702, Purified anti-Siglec-11 Antibody, 100μg","description":"\u003cp\u003eSiglec-11 is a 686 amino acid single-pass type I membrane protein that contains one Ig-like V-type domain and three Ig-like C2-type domains. In humans, Siglec-11 is widely expressed on tissue macrophages, which include microglia in the brain. No expression of Siglec-11 is seen in the brain compartment of non-human primates. Siglec-11 functions as an adhesion molecule that preferentially binds to α-2, 8-linked sialic acid trimers and mediates sialic acid binding to cells. It is a negative regulator of microglia, both related to inflammation and phagocytic function. In murine microglial cells with ectopic expression of human Siglec-11, stimulation of Siglec-11 could induce the transcription of the pro-inflammatory mediator genes, such as IL-1β and nitric oxide synthase-2. Phagocytosis of apoptotic neuronal material has shown to be reduced in Siglec-11 expressing microglia. Co-culture of Siglec-11 expressing microglia and neurons demonstrated neuroprotective functions of Siglec-11.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant Siglec-11 fused to Fc region of human IgG.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Angata T, et al. 2002. J. Biol. Chem. 277:24466.\u003cbr\u003e\nRRID: AB_2566496 (BioLegend Cat. No. 681702)\u003cbr\u003e\nStructure: 698 amino acids, predicted molecular weight of 66 kD. Apparent molecular weight is 100-130 kD due to N-glycosylation.\u003cbr\u003e\nDistribution: Plasma membrane.\u003cbr\u003e\nFunction: Adhesion molecule that mediates sialic-acid dependent binding to cells. The cytosolic domain of Siglec-11 is phosphorylated at tyrosine residue(s) upon pervanadate treatment of cells and then it recruits the protein-tyroisine phosphatases SHP-1 and SHP-2. Such interaction induces inhibitory cellular signals in immune response.\u003cbr\u003e\nInteraction: Interacts with PTPN6\/SHP-1 and PTPN11\/SHIP-2 upon phosphorylation.\u003cbr\u003e\nLigand\/Receptor: α-2,8-linked sialic acid trimers.\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, Siglec Molecules\u003cbr\u003e\nAntigen References: 1. Angata T, et al. 2002. J. Biol. Chem. 277:24466. 2. Hayakawa T, et al. 2005. Science 309:1693. 3. Kopatz J, et al. 2014. Glycobiology 9:794. 4. Wang Y, et al. 2010. J. Neurosci. 30:3482. 5. Linnartz B, et al. 2010. Int. J. Alzheimers Dis. 2010.\u003cbr\u003e\nGene ID: 114132\u003cbr\u003e\nUniProt: View information about Siglec-11 on UniProt.org\u003cbr\u003e\nClone: 4C4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Sialic acid-binding Ig-like lectin 11, Sialic acid-binding lectin 11\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862413398185,"sku":"681702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-681702","provider":"Iright","version":"1.0","type":"link"}