{"product_id":"biolegend-687002","title":"Biolegend, 687002, Purified anti-ERK1 Antibody, 100μg","description":"\u003cp\u003eERK1 is a member of mitogen-activated kinases (MAPKs) of serine\/threonine protein kinases. ERK1 and ERK2 share 85% homology and both are involved in the MAP kinase signaling cascade. ERK1\/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1\/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial human ERK1 recombinant protein (180-379 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested KO\/KD-WB, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry, a concentration range of 1 - 10 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Product was tested in IHC using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween 20\u003cbr\u003e\nRRID: AB_2616892 (BioLegend Cat. No. 687002)\u003cbr\u003e\nStructure: 379 amino acids with a predicted molecular weight of 43.1 kD. Contains a kinase domain responsible for phosphorylation of down stream substrates.\u003cbr\u003e\nDistribution: Nucleus and cytoplasm.\u003cbr\u003e\nFunction: ERK1 is a serine\/threonine kinase which acts as a key component of the MAP kinase signaling pathway. ERK1 and ERK2 have been shown to play redundent roles in cell proliferation, differentiation, migration, survival, and apoptosis.\u003cbr\u003e\nInteraction: Interacts with ADAM15, ARRB2, CANX, DAPK1, HSF4, IER3, MAP2K1\/MEK1, MORG1, NISCH, SGK1, TPR, CDKN2AIP, PEA15, and MKNK2.\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology\u003cbr\u003e\nMolecular Family: Postsynaptic proteins, Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320. 5. Torii S, et al. 2004. J. Biochem. 136:557. 6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.\u003cbr\u003e\nGene ID: 5595\u003cbr\u003e\nUniProt: View information about ERK1 on UniProt.org\u003cbr\u003e\nClone: W15133A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Extracellular signal-regulated kinase 1, Mitogen-activated protein kinase 3 (MAPK3), p44ERK1, p44-MAPK, PRKM3\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866299027625,"sku":"687002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-687002","provider":"Iright","version":"1.0","type":"link"}