{"product_id":"biolegend-688002","title":"Biolegend, 688002, Purified anti-MMP8 Antibody, 100μg","description":"\u003cp\u003eMMP-8, also termed neutrophil collagenase, is a member of the matrix metalloproteinase family proteins (MMPs). Members of this family are zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. Substrates of MMP-8 include type I, II, and III triple-helical collagens, gelatin peptides, proteoglycans, fibronectin, aggrecan, substance P, serpins, β-casein, and angiotensin. MMP-8 is released by neutrophils upon IL-1β, IL-8, GM-CSF and TNF-α stimulation. Besides playing a role in phagocytosis, MMP-8 has been implicated in rheumatoid arthritis (RA) because of its high efficiency in infiltrating connective tissue and breaking down ECM. In fact, serum level of MMP-8 is a strong predictor of mortality in RA, as this is consistent with increased activation of neutrophils in RA. In addition, MMP-8 has been shown to play a crucial role in cardiovascular diseases by promoting atherosclerotic lesion formation. This is supported by the finding that MMP-8 knockout mice have less endothelial cells or plaque angiogenesis in the atherosclerotic plaques. In a clinical study, it was shown that the ratio of MMP-9\/TIMP-1, as well as MMP-8 serum levels, could be a useful predictor of prognosis in patients with hepatocellular carcinomas.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human MMP-8, amino acids (Phe21-Gly467) (Accession# NP_002415.1), is expressed with a C-terminal His tag and a linker sequence in 293E cell lines.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedIHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2617008 (BioLegend Cat. No. 688002)\u003cbr\u003e\nStructure: 467 amino acids, SDS-PAGE apparent molecular weight of Pro-MMP8 is 65-75 kD and Mature MMP8 is 55-60 kD. Consists of a prodomain that is cleaved upon activation, a catalytic domain, a hinge region and a carboxyl terminal domain with hemopexin-like repeats\u003cbr\u003e\nDistribution: Neutrophils, macrophages, and monocytes.\u003cbr\u003e\nInteraction: Collagen type I, II, and III, gelatin, proteoglycans, fibronectin, aggrecan, substance P, serpins, ß-casein, and angiotensin.\u003cbr\u003e\nCell Type: Neutrophils, Macrophages, Monocytes\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: 1. Nagase H, Woessner JF Jr. 1999. J. Biol. Chem. 274:21491. 2. Gill SE, Parks WC. 2008. Int. J. Biochem. Cell Biol. 40:1334. 3. Mattey DL, et al. 2012. Arthritis Res. Ther. 14:R204. 4. Fang C, et al. 2013. Cardiovasc. Res. 99:146. 5. Lempinen M, et al. 2013. Ann. Med. 45:482. 6. Sommer K, et al. 2013. PLoS One 8:e73992.\u003cbr\u003e\nGene ID: 4317\u003cbr\u003e\nUniProt: View information about MMP-8 on UniProt.org\u003cbr\u003e\nClone: 1502022\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Matrix metallopeptidase 8, CLG1, MMP8, PMN-CL, Neutrophil Collagenase, MMP-8\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864350675113,"sku":"688002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-688002","provider":"Iright","version":"1.0","type":"link"}