{"product_id":"biolegend-688302","title":"Biolegend, 688302, Purified anti-Th-POK (ZFP-67) Antibody, 100μg","description":"\u003cp\u003eTh-POK is a member of the BTB-POZ domain-containing zinc finger transcription factor family. It regulates gene expression during intrathymic T cell differentiation. A single autosomal recessive mutation of the Th-POK gene results in the absence of mature CD4 + helper T cells, called helper-deficient (HD) phenotype. In Th-POK deficient mice, MHC class II restricted thymocytes are redirected to the CD8 + T cell lineage. On the contrary, forced expression of Th-POK redirects MHC class I restricted thymocytes to the CD4 + T cell lineage. Inactivation of Th-POK in mature CD4 + T cells results in induction of the genes preferentially expressed in CD8 + T cells, such as CD8, granzyme B, and IFN-γ. These findings suggest that Th-POK is essential in promoting commitment of immature T cells to the CD4 lineage and maintaining the characteristics of mature CD4 + T helper cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse, Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial mouse Th-POK recombinant protein (197-350 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg\/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone recognizes mouse TH-POK. This clone displayed a similar affinity to BioLegend clone 11H11A14 by western blot. However, clone 11H11A14 does not recognize mouse TH-POK.\u003cbr\u003e\nRRID: AB_2617025 (BioLegend Cat. No. 688302)\u003cbr\u003e\nStructure: 539 amino acids with a predicted molecular weight of 58 kD and observed of approximately 70 kD; contains four C2H2-type zinc finger regions that are responsible for DNA binding; contains a BTB-POZ domain which is involved in homodimerization.\u003cbr\u003e\nDistribution: Nucleus.\u003cbr\u003e\nFunction: Transcription factor that regulates CD4 lineage commitment of immature T-cell precursors; acts as a transcriptional repressor of collagen and fibronectin genes.\u003cbr\u003e\nInteraction: Interacts with the acetyltransferase p300.\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Kappes DJ. 2010. Immunol. Rev. 238:182. 2. Mariani F, et al. 2013. PLoS One 8:e54488. 3. Zhang M, et al. 2010. J. Immunol. 185:3960. 4. Egawa T, et al. 2008. Nat. Immunol. 9:1131. 5. Wildt KF, et al. 2007. J. Immunol. 179:4405.\u003cbr\u003e\nGene ID: 51043\u003cbr\u003e\nUniProt: View information about Th-POK on UniProt.org\u003cbr\u003e\nClone: W15147A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T-helper-inducing POZ\/Krueppel-like factor, Zinc finger and BTB domain-containing protein 7B (ZBTB7B), Krueppel-related zinc finger protein (cKrox), Zinc finger protein 67 homolog (ZFP-67), Thpok\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866299846825,"sku":"688302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-688302","provider":"Iright","version":"1.0","type":"link"}