{"product_id":"biolegend-690101","title":"Biolegend, 690101, Purified anti-STAT6 Phospho (Tyr641) Antibody, 25μg","description":"\u003cp\u003eSTAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Tyr641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, translocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human STAT6 peptide phosphorylated at Tyr641.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, ChIP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.05 µg per ml. For immunocytochemistry, a concentration range of 0.2 - 1.0 μg\/ml is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody recognizes STAT6 Phospho (Tyr641) in all three isoforms at 74, 82, and 94 kD. The predominant band detected is at 94 kD.\u003cbr\u003e\nRRID: AB_2715760 (BioLegend Cat. No. 690101) AB_2715760 (BioLegend Cat. No. 690102)\u003cbr\u003e\nStructure: 847 amino acids, predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization.\u003cbr\u003e\nDistribution: Translocates to the nucleus when phosphorylated.\u003cbr\u003e\nFunction: STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling.\u003cbr\u003e\nInteraction: STAT6 interacts with CBP\/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: Nuclear Markers, Phospho-Proteins\u003cbr\u003e\nAntigen References: Goenka S, et al. 2011. Immunol. Res. 50:87 Wurster AL, et al. 2000. Oncogene. 19:2577 Akira S, 1999. Stem Cells. 17:138 Zamorano J, et al. 2005. J. Immunol. 174:2843 David M, et al. 2001. Oncogene. 20:6660 Takeda K, et al. 1996. Nature. 380:627\u003cbr\u003e\nGene ID: 6778\u003cbr\u003e\nUniProt: View information about STAT6 Phospho Tyr641 on UniProt.org\u003cbr\u003e\nClone: A15137A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Signal transducer and activator of transcription 6, IL-4 Stat\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864386850985,"sku":"690101","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-690101","provider":"Iright","version":"1.0","type":"link"}