{"product_id":"biolegend-690451","title":"Biolegend, 690451, Purified anti-STAT3 Phospho (Tyr705) Recombinant Antibody, 25μg","description":"\u003cp\u003eTyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide corresponding to STAT3 phosphorylated at tyrosine 705\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg\/mL is recommended. For flow cytometric staining using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone shows high pan-reactivity to STAT3 when used for WB. BioLegend clone 13A3-1 is more suitable for phospho-specific detection of STAT3. This recombinant antibody clone is derived from clone A16002B.\u003cbr\u003e\nRRID: AB_2894516 (BioLegend Cat. No. 690451) AB_2894516 (BioLegend Cat. No. 690452)\u003cbr\u003e\nStructure: STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.\u003cbr\u003e\nDistribution: Ubiquitous\u003cbr\u003e\nFunction: STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases. The small GTPase Rac1 binds and regulates activity of STAT3.\u003cbr\u003e\nLigand\/Receptor: When activated, STAT3 dimerizes and translocates to the nucleus where it regulates gene expression\u003cbr\u003e\nCell Type: Embryonic Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells\u003cbr\u003e\nMolecular Family: Nuclear Markers, Phospho-Proteins\u003cbr\u003e\nAntigen References: Akira S, et al. 1994. Cell. 77:63. Zhang X, et al. 1995. Science. 267:1990. Sanchez-Margalet V, et al. 2001. Cell Immunol. 211:30. Simon A, et al. 2000. Science. 290:144. Hoey T, et al. 1999. Adv Immunol. 71:145.\u003cbr\u003e\nGene ID: 6774\u003cbr\u003e\nUniProt: View information about STAT3 on UniProt.org\u003cbr\u003e\nClone: A16002B.Rec\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864391536809,"sku":"690451","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-690451","provider":"Iright","version":"1.0","type":"link"}