{"product_id":"biolegend-695702","title":"Biolegend, 695702, Purified anti-Mcl-1 Antibody, 100μg","description":"\u003cp\u003eMyeloid cell leukemia sequence 1 (MCL-1), also known as Bcl2-L-3, is a member of the pro-survival BCL-2 family of apoptotic regulatory proteins. MCL-1 plays a crucial role in the survival and development of various cell types by binding to proapoptotic proteins and inhibiting the mitochondrial apoptotic pathway. Unlike Bcl-2 and Bcl-xL, MCL-1 lacks a BH4 domain and contains an N-terminal PEST region with specific sequences for ubiquitination, phosphorylation, and caspase cleavage sites, which are important in regulating the half-life of MCL-1. Phosphorylation of at T92 and T163 by ERK inhibits MCL-1 turnover, whereas phosphorylation by GSK3β at S159 facilitates proteosomal degradation. Increased expression of MCL-1 is correlated with tumor survival and is associated with resistance of chemotherapeutics.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Full length mouse MCL-1 recombinant protein expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 0.5 - 2.5 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone W16014A does not cross-react with human (in-house tested). This antibody recognizes the two reported isoforms of MCL-1 (MWs: 31 and 35 KDa).\u003cbr\u003e\nRRID: AB_2650880 (BioLegend Cat. No. 695702)\u003cbr\u003e\nStructure: There are two reported isoforms of MCL-1 (MWs: 31 and 35 kDa). The protein contains PEST, BH1, BH2, and BH3 domains.\u003cbr\u003e\nDistribution: Nucleus, Cytoplasm, Mitochondria.\u003cbr\u003e\nFunction: MCL-1 is a prosurvival protein belonging to the BCL-2 family and is required for the survival and development of various cell types.\u003cbr\u003e\nInteraction: MCL-1 forms a homodimer. Interacts with HIF3A, PMAIP1, BBC3, BAX, BAK1, TPT1, BOP, BCL2L11, BAD, BOK, BIK, and BMF.\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology\u003cbr\u003e\nAntigen References: 1. Kozopas K, et al. 1993. P. Natl. Acad. Sci. USA 90:3516. 2. Zhou P, et al. 1997. Blood 89:630. 3. Chao J, et al. 1998. Mol. Cell. Biol. 18:4883. 4. Epling-Burnette P, et al. 2001. J. Immunol. 166:7486. 5. Moulding D, et al. 1998. Blood 92:2495. 6. Inoshita S, et al. 2002. J. Biol. Chem. 277(46):43740. 7. Opferman JT, et al. 2003. Nature. 426:671.\u003cbr\u003e\nGene ID: 4170\u003cbr\u003e\nUniProt: View information about Mcl-1 on UniProt.org\u003cbr\u003e\nClone: W16014A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3 (BCL2-L-3), Bcl-2-related protein EAT\/mcl1,\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866301485225,"sku":"695702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-695702","provider":"Iright","version":"1.0","type":"link"}