{"product_id":"biolegend-695902","title":"Biolegend, 695902, Purified anti-NF-κB p100\/p52 Antibody, 100μg","description":"\u003cp\u003eNuclear factor NF-kappa-B p100\/p52 subunit (NF-κB2) is a member of the Rel\/NF-κB family of transcription factors. NF-κB2 p100 subunit contains an ankyrin repeat domain that has IκB activity to retain NF-κB2 proteins in the cytoplasm. Upon activation, NF-κB2 p100 subunit is processed to p52 subunit through the ubiquitin-proteasome pathway and translocated to the nucleus. NF-κB2 p52 subunit forms homo- or heterodimers with other Rel-like domain-containing proteins, including c-REL, RELA\/p65, RELB, NFKB1\/p105, and NFKB1\/p50, subsequently binds to specific kappa-B sites and regulates expression of their target genes. NF-κB participates in the regulation of peripheral lymphoid development, thymic development, and B cell maturation. Mutations in the NF-κB2 gene lead to defects in B cell development and have been associated with lymphoma and other hematogenous malignancies.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Human NF-κB2 recombinant protein (1-250 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/ml. For immunocytochemistry, a concentration range of 0.2 - 1.0 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone W16082A does not cross-react with mouse (in-house tested).\u003cbr\u003e\nRRID: AB_2650935 (BioLegend Cat. No. 695902)\u003cbr\u003e\nStructure: NF-κB2 p100 subunit contains 900 amino acids with a predicted molecular weight of 96.7 kD. Contains an N-terminal RHD domain for DNA binding and a C-terminal ankyrin repeat domain. NF-κB2 p100 subunit is processed to a p52 subunit upon activation.\u003cbr\u003e\nDistribution: Nucleus and cytoplasm.\u003cbr\u003e\nFunction: NF-κB2 is a transcription factor involved in the regulation of peripheral lymphoid development, thymic development, and B cell maturation.\u003cbr\u003e\nInteraction: Forms homodimers or heterodimers with other Rel-like domain-containing proteins including c-REL, RELA\/p65, RELB, NFKB1\/p105, and NFκB1\/p50. Interacts with BCL3, BTRC, TSC22D3, and MAP3K8.\u003cbr\u003e\nBiology Area: Cell Biology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Lee CE, et al. 2014. Blood. 124:2964. 2. Savinova OV, et al. 2009. Mol Cell. 34:591. 3. Tucker E, et al. 2007. J. Immunol. 179:7514. 4. Kim KE, et al. 2000. Oncogene. 19:1334. 5. Heusch M, et al. 1999. Oncogene. 18:6201. 6. de Wit H, et al. 1998. Leukemia. 12:363.\u003cbr\u003e\nGene ID: 4791\u003cbr\u003e\nUniProt: View information about NF-kappaB p100\/p52 on UniProt.org\u003cbr\u003e\nClone: W16082A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866303582377,"sku":"695902","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-695902","provider":"Iright","version":"1.0","type":"link"}