{"product_id":"biolegend-698101","title":"Biolegend, 698101, Purified anti-NFIL3 Antibody, 25μg","description":"\u003cp\u003eNFIL3, also known as E4BP4, belongs to the basic leucine zipper (bZIP) family of transcription factors. NFIL3 has been shown to bind to the promoter region of the IL-3 gene; IL-3 treatment induces the expression of NFIL3. NFIL3 regulates several immunological processes including Th2 and Th17 cytokine production, Th17 cell differentiation, conventional natural killer (NK) cell differentiation, development of non-NK ILC lineages, and the response of macrophages and dendritic cells. NFIL3 has been found to prevent apoptosis of pro-B lymphocytes and is involved in IgE class switching. NFIL3-deficient mice showed impaired development of NK cells and reduced expression of transcription factors ID2, EOMES, GATA3, and TBX21.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Human NFIL3 recombinant protein (1-250 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2 µg\/mL (1:250-1:1000 diluted). It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2687291 (BioLegend Cat. No. 698101) AB_2687267 (BioLegend Cat. No. 698102)\u003cbr\u003e\nStructure: 462 amino acids with a predicted molecular weight of 50.9 kD. Contains a basic-leucine zipper (bZIP) domain responsible for DNA binding.\u003cbr\u003e\nDistribution: Nucleus\u003cbr\u003e\nFunction: NFIL3 is a transcription factor that regulates a variety of cellular processes in lymphocyte subsets including cytokine production, class switching, and cell differentiation.\u003cbr\u003e\nInteraction: NFIL3 forms a homodimer and interacts with DR1, PER2, and CRY2.\u003cbr\u003e\nAntigen References: 1. Xu W, et al. 2015. Cell Rep. 10:2043. 2. Zhu C, et al. 2015. Nat. Commun. 6:6072. 3. Seillet C, et al. 2014. J. Immunol. 192:2667. 4. Keniry M, et al. 2013. Genes Dev. 27:916. 5. Kashiwada M, et al. 2011. EMBO J. 30:2071. 6. Kobayashi T, et al. 2011. J. Immunol. 186:4649.\u003cbr\u003e\nGene ID: 4783\u003cbr\u003e\nUniProt: View information about NFIL3 on UniProt.org\u003cbr\u003e\nClone: W16121C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL3BP1, E4 binding protein 4 (E4BP4), NF-IL3A, NFIL3A\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866304368809,"sku":"698101","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-698101","provider":"Iright","version":"1.0","type":"link"}