{"product_id":"biolegend-698601","title":"Biolegend, 698601, Purified anti-Centrin 2 (Caltractin) Antibody, 25μg","description":"\u003cp\u003eCentrin, also known as caltractin, was first identified in the green alga Tetraselmis striata and is a member of the highly conserved EF-hand superfamily. Centrins are small calcium binding proteins and play essential roles in the duplication and segregation of microtubule organizing centers (MTOCs). All of the three identified human centrins contain four EF-hand motifs and share high amino acid sequence homology. Human Centrin-1 expression is restricted to male germ cells and retina, while Centrin-2 and Centrin-3 are ubiquitously expressed. In addition to its function in centrosome duplication, Centrin-2 has been found to be present in both cytosol and nucleus. Centrin-2 forms a trimeric complex with the human homolog of Rad23B and the xeroderma pigmentosum group C (XPC) protein. The Centrin-2\/Rad23B\/XPC complex acts as an essential component of the nucleotide excision repair (NER) pathway.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Human Centrin-2 recombinant protein (full length) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested KO\/KD-WB, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml (1:500 - 1:5000 dilution). For immunocytochemistry, the recommended usage is 0.5 - 2.5 µg per mL (1:100 - 1:500 dilution). It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Antibody specificity was confirmed by knock-down of Centrin 2 using siRNA (in-house tested)\u003cbr\u003e\nProduct Citations: St-Denis-Bissonnette F, et al. 2023. J Extracell Vesicles. e12387:12. PubMed Takeda Y, et al. 2024. Nat Commun. 2216:15. PubMed Stojanovic N, et al. 2024. Sci Rep. 26808:14. PubMed\u003cbr\u003e\nRRID: AB_2715793 (BioLegend Cat. No. 698601) AB_2715794 (BioLegend Cat. No. 698602)\u003cbr\u003e\nStructure: 172 amino acids with a predicted molecular weight of 19.7 kD. Contains four EF-hand domains.\u003cbr\u003e\nDistribution: Nucleus. Cytoplasm. Centrosome.\u003cbr\u003e\nFunction: Centrin-2 is a calmodulin-related protein involved in the regulation of centrosome duplication and nucleotide excision repair (NER) of DNA.\u003cbr\u003e\nInteraction: Forms the XPC complex with Rad23B and XPC. Interacts with CCP110 and SFI1.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, DNA Repair\/Replication\u003cbr\u003e\nAntigen References: 1. Sawant DB, et al. 2015. Mol Biol Cell. 26:3741. 2. Ying G, et al. 2014. J Neurosci. 34:6377. 3. Nishi R, et al. 2013. Nucleic Acids Res. 41:6917. 4. Boutros R, et al. 2011. Biol Cell. 103:55. 5. Yang CH, et al. 2010. Mol Biol Cell. 21:4361. 6. Nishi R, et al. 2005. Mol Cell Biol. 25:5664.\u003cbr\u003e\nGene ID: 1069\u003cbr\u003e\nUniProt: View information about Centrin 2 on UniProt.org\u003cbr\u003e\nClone: W16110A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Caltractin isoform 1, CETN2, CALT, CEN2, EF-hand protein 2, 20 kD calcium-binding protein, Centrin-2\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866304237737,"sku":"698601","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-698601","provider":"Iright","version":"1.0","type":"link"}