{"product_id":"biolegend-698701","title":"Biolegend, 698701, Purified anti-TCF12 Antibody, 25μg","description":"\u003cp\u003eTCF12, also known as HTF4 or HEB, is a member of class I basic helix-loop-helix (bHLH) protein family that recognizes the consensus E-box (5'-CANNTG-3') DNA binding site. TCF12 is a transcription factor that participates in regulating gene expression through formation of homodimer or heterodimers with other bHLH E-proteins. TCF12 has been shown to be involved in neuron differentiation and is upregulated in certain cancer cells. TCF12 functions as a transcriptional repressor responsible for down-regulating expression of E-cadherin in colorectal cancer cells. TCF12-deficient mice show abnormality in brain development and a high percentage of postnatal death. Mutations in TCF12 gene have been linked to coronal craniosynostosis.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Human TCF12 recombinant protein (108-521 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml (1:250-1:2500 diluted). It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2687319 (BioLegend Cat. No. 698701) AB_2687320 (BioLegend Cat. No. 698702)\u003cbr\u003e\nStructure: 706 amino acids with a predicted molecular weight of 76 kD. Contains a C-terminal bHLH domain responsible for DNA binding.\u003cbr\u003e\nDistribution: Nucleus\u003cbr\u003e\nFunction: TCF12 is a transcriptional regulator involved in neuronal development and is up-regulated in some type of tumors.\u003cbr\u003e\nInteraction: Forms a homodimer or a heterodimer with myogenin, E12 and ITF2 proteins. Interacts with PTF1A, NEUROD2, RUNX1T1, AML1-MTG8\/ETO, and BHLHA9.\u003cbr\u003e\nBiology Area: Cell Biology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Yi S, et al. 2017. Stem Cells. 35:386. 2. Chen YF, et al. 2016. Cancer Res. 76:4872. 3. Goos JA, et al. 2016. Hum. Mutat. 37:732. 4. Labreche K, et al. 2015. Nat. Commun. 6:7207. 5. Sharma VP, et al. 2013. Nat. Genet. 45:304. 6. Lee CC, et al. 2012. J. Biol. Chem. 287:2798.\u003cbr\u003e\nGene ID: 6938\u003cbr\u003e\nUniProt: View information about TCF12 on UniProt.org\u003cbr\u003e\nClone: W16168A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Transcription factor 12, Class B basic helix-loop-helix protein 20 (bHLHb20), HEB, HTF4\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866306072745,"sku":"698701","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-698701","provider":"Iright","version":"1.0","type":"link"}