{"product_id":"biolegend-699309","title":"Biolegend, 699309, PE anti-Vimentin Antibody, 25μg","description":"\u003cp\u003eVimentin are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is a widely expressed and highly conserved 54 kD protein that is constitutively expressed in mesenchymal cells, endothelial cells lining blood vessels, renal tubular cells, macrophages, neutrophils, fibroblasts, and leukocytes 1,2 . Vimentin is used as a marker of mesenchymal cells to distinguish them from epithelial cells 3 . Increased vimentin expression is frequently used as an EMT marker in cancer 4 . Autoantibodies to vimentin are commonly found in patients with autoimmune diseases such as Lupus 5 and rheumatoid arthritis 6 , and also found after transplantation 7 .\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial mouse Vimentin (224-446 a.a.) recombinant protein expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: This antibody does not cross-react with human (in-house tested). May be weakly reactive with human Vimentin by WB.\u003cbr\u003e\nRRID: AB_2927908 (BioLegend Cat. No. 699309) AB_2927908 (BioLegend Cat. No. 699310)\u003cbr\u003e\nStructure: 466 amino acids with a predicted molecular weight of 54 kD.\u003cbr\u003e\nDistribution: Cytoplasm.\u003cbr\u003e\nFunction: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.\u003cbr\u003e\nInteraction: HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6, RAB8B, TOR1A, TOR1AIP1, and BCAS3.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Intermediate Filaments\u003cbr\u003e\nAntigen References: 1. Kidd ME, et al. 2014. Am J Respir Cell Mol Biol. 50:1. 2. Fuchs E, et al. 1994. Annu Rev Biochem. 63:345. 3. Zeisberg M, et al. 2009. J Clin Invest. 119:1429. 4. Scanlon CS, et al. 2013. J Dent Res. 92:114. 5. Thebault S, et al. 2002. J Immunol. 169:4046. 6. Vossenaar ER, et al. 2004. Arthritis Res Ther. 6:R142. 7. Rose ML. 2013. Hum Immunol. 74:1459.\u003cbr\u003e\nGene ID: 22352\u003cbr\u003e\nUniProt: View information about Vimentin on UniProt.org\u003cbr\u003e\nClone: W16220A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Vim, CTRCT30, HEL 113, Epididymis Luminal Protein 113\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866309677225,"sku":"699309","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-699309","provider":"Iright","version":"1.0","type":"link"}