{"product_id":"biolegend-718554","title":"Biolegend, 718554, Recombinant Human Siglec 10-Fc Chimera (carrier-free), 25μg","description":"\u003cp\u003eSialic acid binding Ig-like lectin (Siglec)-10 is a lectin that specifically binds α-2,3- or α-2,6-linked sialic acid. It is a member of the Ig-superfamily and is expressed on monocytes, eosinophils, neutrophils, B cells, and a subset of NK cells. It functions as an inhibitory receptor, helping to regulate inflammation and prevent excessive immune activation. Siglec-10 is a single pass transmembrane protein. The extracellular domain contains one V-set Ig-like domain and three C2-set domains. The cytoplasmic domain contains one immunoreceptor tyrosine-based inhibitor motif (ITIM). The inhibitory receptor Siglec-10 binds to its ligand CD24 on cancer cells, triggering phosphorylation of the two immunoreceptor tyrosine-based inhibition motifs in its cytoplasmic domain. This activation initiates anti-inflammatory and anti-phagocytic signaling cascades mediated by the phosphatases SHP-1 and SHP-2.  Beyond its interaction with Siglec-10 on innate immune cells, CD24 also suppresses inflammatory responses in various conditions, including infections, sepsis, liver injury, and chronic graft-versus-host disease (GVHD). Siglec-G is the ortholog of human Siglec-10. CD52, CD24, and vascular adhesion protein-1 (VAP-1) have been all described as ligands of Siglec-10.\u003cbr\u003e\n25μg\u003cbr\u003e\nSource: Recombinant human Siglec 10, amino acid Met17-Asn550 (Accession No. Q96LC7) with a C-terminal IEGRMD-human IgG1 (Pro100-Lys330)-Fc tag was expressed in CHO cells.\u003cbr\u003e\nMolecular Mass: The 771 amino acid recombinant protein has a predicted molecular mass of approximately 85.3 kD. The DTT-reduced and non-reduced proteins migrate at approximately 80 - 115 kD and 140-240 kD respectively by SDS-PAGE. The N-terminal amino acid is Met.\u003cbr\u003e\nPurity: \u0026gt; 95%, as determined by Coomassie stained SDS-PAGE.\u003cbr\u003e\nFormulation: 0.22 µm filtered protein solution is in PBS, 10% Glycerol, pH 7.4\u003cbr\u003e\nEndotoxin Level: Less than 0.1 EU per µg protein as determined by the LAL method.\u003cbr\u003e\nConcentration: 25 µg size is bottled at 200 µg\/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg\/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze\/thaw cycles.\u003cbr\u003e\nActivity: Biotinylated recombinant human CD52 binds to immobilized recombinant human Siglec 10 in a dose-dependent manner. The ED50 for this effect is 0.4-2.4 μg\/mL\u003cbr\u003e\nApplication: Biossay\u003cbr\u003e\nApplication Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].\u003cbr\u003e\nStructure: Dimers\u003cbr\u003e\nDistribution: Monocytes, eosinophils, B cells, and a subset of NK cells\u003cbr\u003e\nFunction: Cell adhesion, signal transduction\u003cbr\u003e\nInteraction: SHP-1, PTPN6\u003cbr\u003e\nLigand\/Receptor: CD52, CD24, VAP-1\u003cbr\u003e\nBioactivity: Measured by its ability to bind biotinylated recombinant human CD52\u003cbr\u003e\nCell Type: B cells, Eosinophils, Monocytes, NK cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Siglec Molecules\u003cbr\u003e\nAntigen References: Chen GY, et al. 2009. Science. 323:1722-5. Aizawa H, et al. 2003. Genomics.82:521-30. Kitzig F, et al. 2002. Biochem Biophys Res Commun. 296:355-62. Harrison LC, et al. 2024. Front Immunol. 15:1497889. Bhamidipati K, et al. 2021. Front Immunol. 11:626820. Bandala-Sanchez E, et al. 2018. Proc Natl Acad Sci U S A. 115:7783. Toubai T, et al. 2017. JCI Insight. 2:e92293. Barkal AA, et al. 2019. Nature. 572:39.\u003cbr\u003e\nGene ID: 89790\u003cbr\u003e\nUniProt: View information about Siglec 10 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Sialic acid binding Ig-like lectin 10, Siglec10, Siglec-10, Siglec-likeprotein 2, SLG2, MGC126774, PRO940\u003cbr\u003e\nQ: Why choose BioLegend recombinant proteins?\u003cbr\u003e\nA: • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.\u003cbr\u003e\n     • Greater than 95% Purity or higher, tested on every lot of product.\u003cbr\u003e\n     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.\u003cbr\u003e\n     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.\u003cbr\u003e\n     • Bulk and customization available. Contact us.\u003cbr\u003e\n     • Learn more about our Recombinant Proteins.\u003cbr\u003e\nQ: How does the activity of your recombinant proteins compare to competitors?\u003cbr\u003e\nA: We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!\u003cbr\u003e\nQ: What is the specific activity or ED50 of my recombinant protein?\u003cbr\u003e\nA: The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells\/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.\u003cbr\u003e\nQ: Have your recombinants been tested for stability?\u003cbr\u003e\nA: Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.\u003cbr\u003e\nQ: Does specific activity of a recombinant protein vary between lots?\u003cbr\u003e\nA: Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.\u003cbr\u003e\nQ: How do you convert activity as an ED50 in ng\/ml to a specific activity in Units\/mg?\u003cbr\u003e\nA: Use formula Specific activity (Units\/mg) = 10^6\/ ED50 (ng\/mL)\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864267739305,"sku":"718554","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-718554","provider":"Iright","version":"1.0","type":"link"}