{"product_id":"biolegend-801210","title":"Biolegend, 801210, Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) Antibody, 100μg","description":"\u003cp\u003eTubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: This antibody was raised against microtubules derived from rat brain.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICC - Quality tested ICFC, 3D IHC, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 5.0 µg per million cells. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg\/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, and Western blotting8. This antibody is well characterized and highly reactive to neuron specific Class III ß-tubulin (ßIII). TUJ1 does not identify ß-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC) Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC) Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC) Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB)\u003cbr\u003e\nProduct Citations: Li W, et al. 2022. Nat Commun. 13:7812. PubMed Citro A, et al. 2023. Nat Commun. 14:878. PubMed Arsi\u0026amp;cacute; A, et al. 2020. Sci Rep. 10:6441. PubMed Holman HA, et al. 2019. Front Cell Neurosci. 13:186. PubMed Funato K, et al. 2021. Cell Stem Cell. 28(5):894-905.e7. PubMed Bloom ML, et al. 2020. Chemical Senses. 45(5):333-346. PubMed\u003cbr\u003e\nRRID: AB_2686930 (BioLegend Cat. No. 801209) AB_2686931 (BioLegend Cat. No. 801210)\u003cbr\u003e\nStructure: Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.\u003cbr\u003e\nDistribution: Tissue distribution: central and peripheral nervous system. Cellular distribution: cytosol, cytoskeleton and nucleus.\u003cbr\u003e\nFunction: Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.\u003cbr\u003e\nInteraction: Alpha tubulin, kinesin and dynein.\u003cbr\u003e\nCell Type: Mature Neurons, Neural Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: Microtubules\u003cbr\u003e\nAntigen References: 1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915. 2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987. 3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed 4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101. 5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed\u003cbr\u003e\nGene ID: 10381\u003cbr\u003e\nUniProt: View information about Tubulin beta-3 on UniProt.org\u003cbr\u003e\nClone: TUJ1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864056221865,"sku":"801210","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-801210","provider":"Iright","version":"1.0","type":"link"}