{"product_id":"biolegend-801217","title":"Biolegend, 801217, PE\/Cyanine7 anti-Tubulin β 3 (TUBB3) Antibody, 25tests","description":"\u003cp\u003eTubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: This antibody was raised against microtubules derived from rat brain.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, Western blotting8, and spatial biology (IBEX)9,10. This antibody is well characterized and highly reactive to neuron specific Class III ß-tubulin (ßIII). TUJ1 does not identify ß-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC) Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC) Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC) Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Van Duyne R, et al. 2024. Cells. :13. PubMed\u003cbr\u003e\nRRID: AB_2876747 (BioLegend Cat. No. 801217) AB_2876747 (BioLegend Cat. No. 801218)\u003cbr\u003e\nStructure: Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.\u003cbr\u003e\nDistribution: Tissue distribution: central and peripheral nervous system. Cellular distribution: cytosol, cytoskeleton and nucleus.\u003cbr\u003e\nFunction: Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.\u003cbr\u003e\nInteraction: Alpha tubulin, kinesin and dynein.\u003cbr\u003e\nCell Type: Mature Neurons, Neural Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: Microtubules\u003cbr\u003e\nAntigen References: 1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915. 2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987. 3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed 4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101. 5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed\u003cbr\u003e\nGene ID: 10381\u003cbr\u003e\nUniProt: View information about Tubulin beta-3 on UniProt.org\u003cbr\u003e\nClone: TUJ1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864056352937,"sku":"801217","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-801217","provider":"Iright","version":"1.0","type":"link"}