{"product_id":"biolegend-824806","title":"Biolegend, 824806, PE anti-TREM-2 Antibody, 100μg","description":"\u003cp\u003eTREM-2 is a 26 kD transmembrane glycoprotein that consists of a single extracellular immunoglobulin-like domain, a transmembrane region with a charged lysine residue and a short cytoplasmic tail. It associates with DNAX-activation protein 12 (DAP12) for signaling and function. TREM-2 is expressed on immature monocyte-derived dendritic cells. After activation by microbial products or tumor necrosis factor (TNF) and TNF-related proteins, dendritic cells downregulate the expression of TREM-2. TREM-2 is also expressed by osteoclasts and microglia, where it is involved in bone modeling and brain function, respectively. Another role of TREM-2 might be promoting the removal of apoptotic cells, organic matrix and macromolecules by microglia.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant mouse TREM-2\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Koga T, et al. 3004. Nature. 428:758. (FC, IP)\u003cbr\u003e\nRRID: AB_2941633 (BioLegend Cat. No. 824805) AB_2941633 (BioLegend Cat. No. 824806)\u003cbr\u003e\nStructure: 26 kD transmembrane glycoprotein, member of the immunoglobulin superfamily\u003cbr\u003e\nDistribution: Subset of macrophages, osteoclasts and microglia, immature dendritic cells\u003cbr\u003e\nFunction: Removal of apoptotic cells, organic matrix and macromolecules, bone modeling\u003cbr\u003e\nInteraction: DNAX-activation protein 12 (DAP12)\u003cbr\u003e\nBiology Area: Cell Biology, Neuroinflammation, Neuroscience\u003cbr\u003e\nAntigen References: Leyns CEG, et al. 2019. Nat Neurosci. 22:1217-22. Zhong L, et al. 2019. Nat Commun. 10:1365. Parhizkar S, et al. 2019. Nat Neurosci. 22:191-204. Filipello F, et al. 2018. Immunity. 48:979-91.\u003cbr\u003e\nGene ID: 83433\u003cbr\u003e\nUniProt: View information about TREM2 on UniProt.org\u003cbr\u003e\nClone: 6E9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Triggering receptor expressed on myeloid cells 2, Trem, TREM-2, Trem2a, Trem2b, Trem2c, TREM2\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862479327401,"sku":"824806","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-824806","provider":"Iright","version":"1.0","type":"link"}