{"product_id":"biolegend-837704","title":"Biolegend, 837704, Purified anti-Neurofilament H \u0026 M (NF-H\/NF-M), Phosphorylated Antibody, 100μg","description":"\u003cp\u003eNeurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and regulate axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE: the light or lowest (NF-L) runs at 68-70 kD; the medium or middle (NF-M) runs at about 145-160 kD; the heavy or highest (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from glia (negative for NF).\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: IHC-P - Quality tested WB - Verified IHC-F, ICC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: For immunohistochemistry, a concentration range of 0.5 - 10 μg\/ml is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Western blotting4, immunocytochemistry2, immunohistochemical staining of frozen tissue sections1,3, and spatial biology (IBEX)5,6. Clone SMI 310 reacts with an extensively phosphorylated epitope of neurofilament H and, to a much lesser extent, neurofilament M in most mammalian species. Phosphatase treatment of samples abolishes reaction with SMI 310. A very extensive degree of hyperphosphorylation of neurofilaments seems to be necessary for its reactivity.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Dubourg O, et al. 2011. Acta Myol. 30(2):103. (IHC-F) PubMed Mizui T, et al. 2009. J Neurochem. 109(2):611. (ICC) Denlger-Crish C, et al. 2014. Front Neursci. 8:290. (IHC-F) PubMed Mulot SF, et al. 1994. FEBS Lett. 349(3):359. (WB) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nRRID: AB_2715865 (BioLegend Cat. No. 837703) AB_2566638 (BioLegend Cat. No. 837704)\u003cbr\u003e\nStructure: The medium or middle neurofilament (NF-M) runs at 145-160 kD, and the heavy or highest neurofilament (NF-H) runs at 200-220 kD.\u003cbr\u003e\nDistribution: Tissue distribution: CNS, peripheral nerves and glandular cells of the prostate. Cellular distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion.\u003cbr\u003e\nFunction: Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.\u003cbr\u003e\nInteraction: Cell bodies and dendrites are generally unstained while other cells and tissues are unreactive, except for peripheral axons.\u003cbr\u003e\nCell Type: Mature Neurons\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Intermediate Filaments, Phospho-Proteins\u003cbr\u003e\nAntigen References: Siedler D, et al. 2014. Front Cell Neurosci. 8:429.\u003cbr\u003e\nGene ID: 4744\u003cbr\u003e\nUniProt: View information about Neurofilament HM NF-H NF-M Phospho on UniProt.org\u003cbr\u003e\nClone: SMI 310\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866295423145,"sku":"837704","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-837704","provider":"Iright","version":"1.0","type":"link"}