{"product_id":"biolegend-838101","title":"Biolegend, 838101, Anti-CD171 (L1CAM) Antibody (Previously Covance catalog# MMS-172R), 500microl","description":"\u003cp\u003eL1CAM, also known as CD171, is a neuronal cell adhesion molecule involved in axon guidance and cell migration with a strong implication in treatment-resistant cancers. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration, and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). CD171 has been shown to function as a cell adhesion molecule mediating homotypic and heterotypic cell-cell interactions in neuronal myelination, neurite outgrowth and regeneration.\u003cbr\u003e\n500microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: This monoclonal antibody was raised against purified chick L1, and binds to the L1 cytoplasmic domain at about T1172 (adjacent to the YRSL sorting signal).\u003cbr\u003e\nPreparation: Ascites\u003cbr\u003e\nConcentration: The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg\/ml.\u003cbr\u003e\nStorage \u0026amp; Handling: Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw.\u003cbr\u003e\nApplication: WB - Quality testedICC, IP, IHC-P - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution for this reagent is 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kamiguchi H, et al. 1998. J. Neurosci. 18(14):5311-21. Lemmon V, et al. 1989. Neuron. 2(6):1597-603. Yip PM, Siu CH. 2001. J. Neurochem. 76(5):1552-64. Schaefer AW, et. al. 2002. J. Cell Biol. 157:1223. (WB, IHC-P, IP) Beer S, et. al. 1999. J. Cell Sci. 112:2667. (IP) Itoh K, et. al. 2005. Mol. Cell Neurosci. 29:245. (WB, IP) Zheng XJ, et. al. 2005. Environ. Technol. 26:1073. (ICC) Chen SY, et. al. 2001. FASEB. J. 15:1649. (IHC-P) PubMed Itoh K, et. al. 2004. J. Cell Biol. 165:145. (WB) Cohen NR, et. al. 1998. Curr. Biol. 8:26. (WB)\u003cbr\u003e\nProduct Citations: Chen S, et al. 2001. FASEB J. 15:1649-1561. PubMed Hu J, et al. 2022. Cell Death Dis. 13:48. PubMed Kleene R, et al. 2021. J Neurochem. 157:1102. PubMed Girbes Minguez M, et al. 2020. FASEB J. 34:9869. PubMed\u003cbr\u003e\nRRID: AB_2565388 (BioLegend Cat. No. 838101)\u003cbr\u003e\nStructure: Expected MW: 200-220 kD\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules\u003cbr\u003e\nGene ID: 3897\u003cbr\u003e\nUniProt: View information about CD171 on UniProt.org\u003cbr\u003e\nClone: 74-5H7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Neural cell adhesion molecule L1, antigen identified by monoclonal antibody R1\u003cbr\u003e\nPreviously: Covance Catalog# MMS-172R\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862483587241,"sku":"838101","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-838101","provider":"Iright","version":"1.0","type":"link"}