{"product_id":"biolegend-846502","title":"Biolegend, 846502, Purified anti-complement C3\/C3b\/iC3b Antibody, 100μg","description":"\u003cp\u003eComplement component 3 (C3) is a 185 kD glycoprotein composed of two chains linked by a disulfide bond. It is the fourth complement component to react in the classical pathway and is a key protein in the alternative and the lectin complement activation pathways. C3b is proteolytically generated from C3 by cleavage of the C3a-C3b peptide bond in the protein by C3 convertase. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils recognize C3b by the complement receptor 1 (CR1, CD35). Opsonization of target surfaces with C3b is central to all three pathways of complement activation. The proteolytically inactive forms of C3b, iC3b, are generated by cleavage of the alpha chain at one or two positions by factor I in the presence of co-factors, such as factor H. A ~2 kD fragment of C3b, C3f, is generated when C3b is cleaved at two positions by factor I. Although iC3b is less active than C3b, iC3b’s interactions with lymphoid and phagocytic cells via CR2 (CD21) and CR3 (CD11b\/CD18) can expand target-specific B and T cells. iC3b, can be cleaved to form C3c and C3dg. Further proteolytic cleavage of C3dg generates C3d and C3g.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human C3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedFC, Direct ELISA - VerifiedELISA Detection - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For flow cytometric staining, the suggested use of this reagent is 1.0 - 2.0 µg per million cells in 100 µl volume. For Direct ELISA, the suggested use of this reagent is 0.01 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody recognizes human C3, C3b and iC3b, and does not cross-react with C3d.Personal communication from the developer of clone 6C9\/C3b indicates it recognizes mouse and non-human primate C3, C3b, and iC3b.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Shi J, et al. 2014. Blood. 123:4015. (FC)\u003cbr\u003e\nRRID: AB_2572183 (BioLegend Cat. No. 846502)\u003cbr\u003e\nStructure: C3 is a 185 kD glycoprotein that is cleaved to generate a 176 kD protein fragment, C3b. iC3b is proteolytically derived from C3b and exists as a mixture of 176 and 174 kD proteins.\u003cbr\u003e\nDistribution: Extracellular, plasma membrane, and serum.\u003cbr\u003e\nFunction: C3b is involved in all three complement pathways and is essential for effective complement activation and presentation of antigens to cells of the adaptive immune system. iC3b is less active than C3b, but the target-bound protein can expand target-specific B-cell and T-cell populations by interacting with lymphoid and phagocytic cells.\u003cbr\u003e\nInteraction: Immune cells interact with C3b and iC3b using CR1 and CR2\/CR3, respectively.\u003cbr\u003e\nLigand\/Receptor: C3b can bind to Factor B, Factor P, Factor H, Factor I, C5, DAF (CD55), MCP (CD46), and CR1 (CD35). iC3b interacts with CR2 (CD21) and CR3 (CD11b\/CD18).\u003cbr\u003e\nBiology Area: Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nAntigen References: 1. Lambris JD, et al. 1988. Immunol. Today 9:387-93.\u003cbr\u003e\nGene ID: 718\u003cbr\u003e\nUniProt: View information about C3\/C3b\/iC3b on UniProt.org\u003cbr\u003e\nClone: 6C9\/C3b\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Complement Component 3, C3b, iC3b, C3bi\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862474707113,"sku":"846502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-846502","provider":"Iright","version":"1.0","type":"link"}